Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBCK / TBCKL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108418-100
Catalog Number: LS-G108418
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSPC302(TBCK) (NM_033115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TBCK | TBCKL | TBC1 domain containing kinase | HSPC302
- Origin Species
- Human
- NCBI GenBank
- NM_033115
- SwissProt
- Q8TEA7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFPLKDAEMG AFTFFASALP HDVCGSNGLP LTPNSIKILG RFQILKTITH PRLCQYVDIS61RGKHERLVVV AEHCERSLED LLRERKPVRY PSYLAPEVIA QGIFKTTDHM PSKKPLPSGP121KSDVWSLGII LFELCVGRKL FQSLDISERL KFLLTLDCVD DTLIVLAEEH GCLDIIKELP181ETVIDLLNKC LTFHPSKRPT PDELMKDKVF SEVSPLYTPF TKPASLFSSS LRCADLTLPE241DISQLCKDIN NDYLAERSIE EVYYLWCLAG GDLEKELVNK EIIRSKPPIC TLPNFLFEDG301ESFGQGRDRS SLLDDTTVTL SLCQLRNRLK DVGGEAFYPL LEDDQSNLPH SNSNNELSAA361ATLPLIIREK DTEYQLNRII LFDRLLKAYP YKKNQIWKEA RVDIPPLMRG LTWAALLGVE421GAIHAKYDAI DKDTPIPTDR QIEVDIPRCH QYDELLSSPE GHAKFRRVLK AWVVSHPDLV481YWQGLDSLCA PFLYLNFNNE ALAYACMSAF IPKYLYNFFL KDNSHVIQEY LTVFSQMIAF541HDPELSNHLN EIGFIPDLYA IPWFLTMFTH VFPLHKIFHL WDTLLLGNSS FPFCIGVAIL601QQLRDRLLAN GFNECILLFS DLPEIDIERC VRESINLFCW TPKSATYRQH AQPPKPSSDS661SGGRSSAPYF SAECPDPPKT DLSRESIPLN DLKSEVSPRI SAEDLIDLCE LTVTGHFKTP721SKKTKSSKPK LLVVDIRNSE DFIRGHISGS INIPFSAAFT AEGELTQGPY TAMLQNFKGK781VIVIVGHVAK HTAEFAAHLV KMKYPRICIL DGGINKIKPT GLLTIPSPQITRTRPLEQKLISEEDLAANDILDYKDDDDKV
830 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.