Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBC1D31 / WDR67 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106637-100
Catalog Number: LS-G106637
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR67(TBC1D31) (NM_145647), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gm85 | WD repeat domain 67 | WDR67
- Origin Species
- Human
- NCBI GenBank
- NM_001145088
- SwissProt
- Q96DN5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 124 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQSTDLGNKE SGKIWHRKPS PATRDGIIVN IIHNTSDYHP KVLRFLNVAF DGTGDCLIAG61DHQGNIYVFD LHGNRFNLVQ RTAQACTALA FNLRRKSEFL VALADYSIKC FDTVTKELVS121WMRGHESSVF SISVHASGKY AITTSSDTAQ LWDLDTFQRK RKLNIRQSVG IQKVFFLPLS181NTILSCFKDN SIFAWECDTL FCKYQLPAPP ESSSILYKVF AVTRDGRILA AGGKSNHLHL241WCLEARQLFR IIQMPTKVRA IRHLEFLPDS FDAGSNQVLG VLSQDGIMRF INMQTCKLLF301EIGSLDEGIS SSAISPHGRY IASIMENGSL NIYSVQALTQ EINKPPPPLV KVIEDLPKNK361LSSSDLKMKV TSGRVQQPAK SRESKMQTRI LKQDLTGDFE SKKNELPDGL NKKRLQILLK421GYGEYPTKYR MFIWRSLLQL PENHTAFSTL IDKGTHVAFL NLQKKYPIKS RKLLRVLQRT481LSALAHWSVI FSDTPYLPLL AFPFVKLFQN NQLICFEVIA TLIINWCQHW FEYFPNPPIN541ILSMIENVLA FHDKELLQHF IDHDITSQLY AWPLLETVFS EVLTREEWLK LFDNIFSNHP601SFLLMTVVAY NICSRTPLLS CNLKDDFEFF FHHRNNLDIN VVIRQVYHLM ETTPTDIHPD661SMLNVFVALT KGQYPVFNQY PKFIVDYQTQ ERERIRNDEL DYLRERQTVE DMQAKVDQQR721VEDEAWYQKQ ELLRKAEETR REMLLQEEEK MIQQRQRLAA VKRELKVKEM HLQDAARRRF781LKLQQDQQEM ELRRLDDEIG RKVYMRDREI AATARDLEMR QLELESQKRL YEKNLTENQE841ALAKEMRADA DAYRRKVDLE EHMFHKLIEA GETQSQKTQK VIKENLAKAE QACLNTDWQI901QSLHKQKCDD LQRNKCYQEV AKLLRENRRK EIEIINAMVE EEAKKWKEAE GKEFRLRSAK961KASALSDASR KWFLKQEINA AVEHAENPCH KEEPRFQNEQ DSSCLPRTSQ LNDSSEMDPS1021TQISLNRRAV EWDTTGQNLI KKVRNLRQRL TARARHRCQT PHLLAATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1066 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.