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Human TBC1D31 / WDR67 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TBC1D31 / WDR67 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TBC1D31 antibodies →
$153.00each
Catalog Number: LS-G95384-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human WDR67(TBC1D31) (NM_145647) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Gm85 | WD repeat domain 67 | WDR67
Origin Species
Human
NCBI GenBank
NM_001145088
SwissProt
Q96DN5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQSTDLGNKE SGKIWHRKPS PATRDGIIVN IIHNTSDYHP KVLRFLNVAF DGTGDCLIAG
61DHQGNIYVFD LHGNRFNLVQ RTAQACTALA FNLRRKSEFL VALADYSIKC FDTVTKELVS
121WMRGHESSVF SISVHASGKY AITTSSDTAQ LWDLDTFQRK RKLNIRQSVG IQKVFFLPLS
181NTILSCFKDN SIFAWECDTL FCKYQLPAPP ESSSILYKVF AVTRDGRILA AGGKSNHLHL
241WCLEARQLFR IIQMPTKVRA IRHLEFLPDS FDAGSNQVLG VLSQDGIMRF INMQTCKLLF
301EIGSLDEGIS SSAISPHGRY IASIMENGSL NIYSVQALTQ EINKPPPPLV KVIEDLPKNK
361LSSSDLKMKV TSGRVQQPAK SRESKMQTRI LKQDLTGDFE SKKNELPDGL NKKRLQILLK
421GYGEYPTKYR MFIWRSLLQL PENHTAFSTL IDKGTHVAFL NLQKKYPIKS RKLLRVLQRT
481LSALAHWSVI FSDTPYLPLL AFPFVKLFQN NQLICFEVIA TLIINWCQHW FEYFPNPPIN
541ILSMIENVLA FHDKELLQHF IDHDITSQLY AWPLLETVFS EVLTREEWLK LFDNIFSNHP
601SFLLMTVVAY NICSRTPLLS CNLKDDFEFF FHHRNNLDIN VVIRQVYHLM ETTPTDIHPD
661SMLNVFVALT KGQYPVFNQY PKFIVDYQTQ ERERIRNDEL DYLRERQTVE DMQAKVDQQR
721VEDEAWYQKQ ELLRKAEETR REMLLQEEEK MIQQRQRLAA VKRELKVKEM HLQDAARRRF
781LKLQQDQQEM ELRRLDDEIG RKVYMRDREI AATARDLEMR QLELESQKRL YEKNLTENQE
841ALAKEMRADA DAYRRKVDLE EHMFHKLIEA GETQSQKTQK VIKENLAKAE QACLNTDWQI
901QSLHKQKCDD LQRNKCYQEV AKLLRENRRK EIEIINAMVE EEAKKWKEAE GKEFRLRSAK
961KASALSDASR KWFLKQEINA AVEHAENPCH KEEPRFQNEQ DSSCLPRTSQ LNDSSEMDPS
1021TQISLNRRAV EWDTTGQNLI KKVRNLRQRL TARARHRCQT PHLLAA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1066 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.