Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TAS1R2 / T1R2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102342-100
Catalog Number: LS-G102342
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R2 (NM_152232), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 71 | GPR71 | GPCRB4 | T1R2 | Taste receptor type 1 member 2 | TR2 | Sweet taste receptor T1R2 | G-protein coupled receptor 71 | TAS1R2
- Origin Species
- Human
- NCBI GenBank
- NM_152232
- SwissProt
- Q8TE23
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPRAKTICS LFFLLWVLAE PAENSDFYLP GDYLLGGLFS LHANMKGIVH LNFLQVPMCK61EYEVKVIGYN LMQAMRFAVE EINNDSSLLP GVLLGYEIVD VCYISNNVQP VLYFLAHEDN121LLPIQEDYSN YISRVVAVIG PDNSESVMTV ANFLSLFLLP QITYSAISDE LRDKVRFPAL181LRTTPSADHH VEAMVQLMLH FRWNWIIVLV SSDTYGRDNG QLLGERVARR DICIAFQETL241PTLQPNQNMT SEERQRLVTI VDKLQQSTAR VVVVFSPDLT LYHFFNEVLR QNFTGAVWIA301SESWAIDPVL HNLTELGHLG TFLGITIQSV PIPGFSEFRE WGPQAGPPPL SRTSQSYTCN361QECDNCLNAT LSFNTILRLS GERVVYSVYS AVYAVAHALH SLLGCDKSTC TKRVVYPWQL421LEEIWKVNFT LLDHQIFFDP QGDVALHLEI VQWQWDRSQN PFQSVASYYP LQRQLKNIQD481ISWHTVNNTI PMSMCSKRCQ SGQKKKPVGI HVCCFECIDC LPGTFLNHTE DEYECQACPN541NEWSYQSETS CFKRQLVFLE WHEAPTIAVA LLAALGFLST LAILVIFWRH FQTPIVRSAG601GPMCFLMLTL LLVAYMVVPV YVGPPKVSTC LCRQALFPLC FTICISCIAV RSFQIVCAFK661MASRFPRAYS YWVRYQGPYV SMAFITVLKM VIVVIGMLAT GLSPTTRTDP DDPKITIVSC721NPNYRNSLLF NTSLDLLLSV VGFSFAYMGK ELPTNYNEAK FITLSMTFYF TSSVSLCTFM781SAYSGVLVTI VDLLVTVLNL LAISLGYFGP KCYMILFYPE RNTPAYFNSM IQGYTMRRDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
839 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.