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Human TAS1R2 / T1R2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TAS1R2 / T1R2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G92297-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R2 (NM_152232) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 71 | GPR71 | GPCRB4 | T1R2 | Taste receptor type 1 member 2 | TR2 | Sweet taste receptor T1R2 | G-protein coupled receptor 71 | TAS1R2
Origin Species
Human
NCBI GenBank
NM_152232
SwissProt
Q8TE23

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPRAKTICS LFFLLWVLAE PAENSDFYLP GDYLLGGLFS LHANMKGIVH LNFLQVPMCK
61EYEVKVIGYN LMQAMRFAVE EINNDSSLLP GVLLGYEIVD VCYISNNVQP VLYFLAHEDN
121LLPIQEDYSN YISRVVAVIG PDNSESVMTV ANFLSLFLLP QITYSAISDE LRDKVRFPAL
181LRTTPSADHH VEAMVQLMLH FRWNWIIVLV SSDTYGRDNG QLLGERVARR DICIAFQETL
241PTLQPNQNMT SEERQRLVTI VDKLQQSTAR VVVVFSPDLT LYHFFNEVLR QNFTGAVWIA
301SESWAIDPVL HNLTELGHLG TFLGITIQSV PIPGFSEFRE WGPQAGPPPL SRTSQSYTCN
361QECDNCLNAT LSFNTILRLS GERVVYSVYS AVYAVAHALH SLLGCDKSTC TKRVVYPWQL
421LEEIWKVNFT LLDHQIFFDP QGDVALHLEI VQWQWDRSQN PFQSVASYYP LQRQLKNIQD
481ISWHTVNNTI PMSMCSKRCQ SGQKKKPVGI HVCCFECIDC LPGTFLNHTE DEYECQACPN
541NEWSYQSETS CFKRQLVFLE WHEAPTIAVA LLAALGFLST LAILVIFWRH FQTPIVRSAG
601GPMCFLMLTL LLVAYMVVPV YVGPPKVSTC LCRQALFPLC FTICISCIAV RSFQIVCAFK
661MASRFPRAYS YWVRYQGPYV SMAFITVLKM VIVVIGMLAT GLSPTTRTDP DDPKITIVSC
721NPNYRNSLLF NTSLDLLLSV VGFSFAYMGK ELPTNYNEAK FITLSMTFYF TSSVSLCTFM
781SAYSGVLVTI VDLLVTVLNL LAISLGYFGP KCYMILFYPE RNTPAYFNSM IQGYTMRRD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.