Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TACR3 / NK3R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119355-100
Catalog Number: LS-G119355
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neurokinin 3 Receptor NK3(TACR3) (NM_001059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HH11 | Nmk-r | NK3R | NKR | TACR3 | Tachykinin receptor 3 | Neurokinin B | Neurokinin B receptor | NK-3 receptor | NK-3R | TAC3R | Neurokinin 3 receptor | Neurokinin beta receptor | Neuromedin-K receptor | TAC3RL
- Origin Species
- Human
- NCBI GenBank
- NM_001059
- SwissProt
- P29371
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATLPAAETW IDGGGGVGAD AVNLTASLAA GAATGAVETG WLQLLDQAGN LSSSPSALGL61PVASPAPSQP WANLTNQFVQ PSWRVALWSL AYGVVVAVAV LGNLIVIWII LAHKRMRTVT121NYFLVNLAFS DASMAAFNTL VNFIYALHSE WYFGANYCRF QNFFPITAVF ASIYSMTAIA181VDRYMAIIDP LKPRLSATAT KIVIGSIWIL AFLLAFPQCL YSKTKVMPGR TLCFVQWPEG241PKQHFTYHII VIILVYCFPL IIMGITYTIV GITLWGGEIP GDTCDKYHEQ LKAKRKVVKM301MIIVVMTFAI CWLPYHIYFI LTAIYQQLNR WKYIQQVYLA SFWLAMSSTM YNPIIYCCLN361KRFRAGFKRA FRWCPFIKVS SYDELELKTT RFHPNRQSSM YTVTRMESMT VVFDPNDADT421TRSSRKKRAT PRDPSFNGCS RRNSKSASAT SSFISSPYTS VDEYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.