LSBio
TACC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TACC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TACC1 antibodies →
$504.00each
Catalog Number: LS-G128276-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TACC1 (NM_001146216), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ga55 | Gastric cancer antigen Ga55 | KIAA1103 | TACC1 | Taxin-1
Origin Species
Human
NCBI GenBank
NM_006283
SwissProt
O75410

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEGSMGVTL EASAEADLKA GNSCPELVPS RRSKLRKPKP VPLRKKAIGG EFSDTNAAVE
61GTPLPKASYH FSPEELDENT SPLLGDARFQ KSPPDLKETP GTLSSDTNDS GVELGEESRS
121SPLKLEFDFT EDTGNIEARK ALPRKLGRKL GSTLTPKIQK DGISKSAGLE QPTDPVARDG
181PLSQTSSKPD PSQWESPSFN PFGSHSVLQN SPPLSSEGSY HFDPDNFDES MDPFKPTTTL
241TSSDFCSPTG NHVNEILESP KKAKSRLITS GCKVKKHETQ SLALDACSRD EGAVISQISD
301ISNRDGHATD EEKLASTSCG QKSAGAEVKG EPEEDLEYFE CSNVPVSTIN HAFSSSEAGI
361EKETCQKMEE DGSTVLGLLE SSAEKAPVSV SCGGESPLDG ICLSESDKTA VLTLIREEII
421TKEIEANEWK KKYEETRQEV LEMRKIVAEY EKTIAQMIED EQRTSMTSQK SFQQLTMEKE
481QALADLNSVE RSLSDLFRRY ENLKGVLEGF KKNEEALKKC AQDYLARVKQ EEQRYQALKI
541HAEEKLDKAN EEIAQVRTKA KAESAALHAG LRKEQMKVES LERALQQKNQ EIEELTKICD
601ELIAKLGKTD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.