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Human TACC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TACC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TACC1 antibodies →
$153.00each
Catalog Number: LS-G83260-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TACC1 (NM_001146216) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ga55 | Gastric cancer antigen Ga55 | KIAA1103 | TACC1 | Taxin-1
Origin Species
Human
NCBI GenBank
NM_006283
SwissProt
O75410

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEGSMGVTL EASAEADLKA GNSCPELVPS RRSKLRKPKP VPLRKKAIGG EFSDTNAAVE
61GTPLPKASYH FSPEELDENT SPLLGDARFQ KSPPDLKETP GTLSSDTNDS GVELGEESRS
121SPLKLEFDFT EDTGNIEARK ALPRKLGRKL GSTLTPKIQK DGISKSAGLE QPTDPVARDG
181PLSQTSSKPD PSQWESPSFN PFGSHSVLQN SPPLSSEGSY HFDPDNFDES MDPFKPTTTL
241TSSDFCSPTG NHVNEILESP KKAKSRLITS GCKVKKHETQ SLALDACSRD EGAVISQISD
301ISNRDGHATD EEKLASTSCG QKSAGAEVKG EPEEDLEYFE CSNVPVSTIN HAFSSSEAGI
361EKETCQKMEE DGSTVLGLLE SSAEKAPVSV SCGGESPLDG ICLSESDKTA VLTLIREEII
421TKEIEANEWK KKYEETRQEV LEMRKIVAEY EKTIAQMIED EQRTSMTSQK SFQQLTMEKE
481QALADLNSVE RSLSDLFRRY ENLKGVLEGF KKNEEALKKC AQDYLARVKQ EEQRYQALKI
541HAEEKLDKAN EEIAQVRTKA KAESAALHAG LRKEQMKVES LERALQQKNQ EIEELTKICD
601ELIAKLGKTD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.