Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
T1R3 / TAS1R3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106428-100
Catalog Number: LS-G106428
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R3 (NM_152228), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TAS1R3 | Taste receptor type 1 member 3 | TR3 | Sweet taste receptor T1R3 | T1R3
- Origin Species
- Human
- NCBI GenBank
- AX239647
- SwissProt
- Q7RTX0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP61VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA121GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF181PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE241GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS301EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG361LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP421VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT481ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF541CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ601ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV661ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV721HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGRYNR ARGLTFAMLA YFITWVSFVP781LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ841NDGNTGNQGK HETRTRPLEQKLISEEDLAANDILDYKDDDDKV
852 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.