T1R3 / TAS1R3 Antibody
Catalog Number: LS-C829264-100
100 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Common Marmoset, Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human T1R3 / TAS1R3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G88830-20
Catalog Number: LS-G88830
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R3 (NM_152228) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TAS1R3 | Taste receptor type 1 member 3 | TR3 | Sweet taste receptor T1R3 | T1R3
- Origin Species
- Human
- NCBI GenBank
- AX239647
- SwissProt
- Q7RTX0
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP61VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA121GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF181PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE241GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS301EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG361LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP421VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT481ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF541CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ601ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV661ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV721HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGRYNR ARGLTFAMLA YFITWVSFVP781LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ841NDGNTGNQGK HETRTRPLEQKLISEEDLAANDILDYKDDDDKV
852 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
