Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
T1R1 / TAS1R1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107259-100
Catalog Number: LS-G107259
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R1 (NM_138697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 70 | GPR70 | GM148 | GPCRB3 | TAS1R1 | G protein-coupled receptor 70 | GPCR-B3 | T1R1 | Taste receptor type 1 member 1 | TR1
- Origin Species
- Human
- NCBI GenBank
- NM_138697
- SwissProt
- Q7RTX1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT61LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS121LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMISYA ASSETLSVKR181QYPSFLRTIP NDKYQVETMV LLLQKFGWTW ISLVGSSDDY GQLGVQALEN QATGQGICIA241FKDIMPFSAQ VGDERMQCLM RHLAQAGATV VVVFSSRQLA RVFFESVVLT NLTGKVWVAS301EAWALSRHIT GVPGIQRIGM VLGVAIQKRA VPGLKAFEEA YARADKEAPR PCHKGSWCSS361NQLCRECQAF MAHTMPKLKA FSMSSAYNAY RAVYAVAHGL HQLLGCASGA CSRGRVYPWQ421LLEQIHKVHF LLHKDTVAFN DNRDPLSSYN IIAWDWNGPK WTFTVLGSST WSPVQLNINE481TKIQWHGKDN QVPKSVCSSD CLEGHQRVVT GFHHCCFECV PCGAGTFLNK SDLYRCQPCG541KEEWAPEGSQ TCFPRTVVFL ALREHTSWVL LAANTLLLLL LLGTAGLFAW HLDTPVVRSA601GGRLCFLMLG SLAAGSGSLY GFFGEPTRPA CLLRQALFAL GFTIFLSCLT VRSFQLIIIF661KFSTKVPTFY HAWVQNHGAG LFVMISSAAQ LLICLTWLVV WTPLPAREYQ RFPHLVMLEC721TETNSLGFIL AFLYNGLLSI SAFACSYLGK DLPENYNEAK CVTFSLLFNF VSWIAFFTTA781SVYDGKYLPA ANMMAGLSSL SSGFGGYFLP KCYVILCRPD LNSTEHFQAS IQDYTRRCGS841TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
841 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.