T1R1 / TAS1R1 Antibody
Catalog Number: LS-C496867
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human T1R1 / TAS1R1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G92291-20
Catalog Number: LS-G92291
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TAS1R1 (NM_138697) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 70 | GPR70 | GM148 | GPCRB3 | TAS1R1 | G protein-coupled receptor 70 | GPCR-B3 | T1R1 | Taste receptor type 1 member 1 | TR1
- Origin Species
- Human
- NCBI GenBank
- NM_138697
- SwissProt
- Q7RTX1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT61LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS121LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMISYA ASSETLSVKR181QYPSFLRTIP NDKYQVETMV LLLQKFGWTW ISLVGSSDDY GQLGVQALEN QATGQGICIA241FKDIMPFSAQ VGDERMQCLM RHLAQAGATV VVVFSSRQLA RVFFESVVLT NLTGKVWVAS301EAWALSRHIT GVPGIQRIGM VLGVAIQKRA VPGLKAFEEA YARADKEAPR PCHKGSWCSS361NQLCRECQAF MAHTMPKLKA FSMSSAYNAY RAVYAVAHGL HQLLGCASGA CSRGRVYPWQ421LLEQIHKVHF LLHKDTVAFN DNRDPLSSYN IIAWDWNGPK WTFTVLGSST WSPVQLNINE481TKIQWHGKDN QVPKSVCSSD CLEGHQRVVT GFHHCCFECV PCGAGTFLNK SDLYRCQPCG541KEEWAPEGSQ TCFPRTVVFL ALREHTSWVL LAANTLLLLL LLGTAGLFAW HLDTPVVRSA601GGRLCFLMLG SLAAGSGSLY GFFGEPTRPA CLLRQALFAL GFTIFLSCLT VRSFQLIIIF661KFSTKVPTFY HAWVQNHGAG LFVMISSAAQ LLICLTWLVV WTPLPAREYQ RFPHLVMLEC721TETNSLGFIL AFLYNGLLSI SAFACSYLGK DLPENYNEAK CVTFSLLFNF VSWIAFFTTA781SVYDGKYLPA ANMMAGLSSL SSGFGGYFLP KCYVILCRPD LNSTEHFQAS IQDYTRRCGS841TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
841 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
