Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYNPO / Synaptopodin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124187-100
Catalog Number: LS-G124187
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Synaptopodin(SYNPO) (NM_001109974), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1029 | SYNPO | Synaptopodin
- Origin Species
- Human
- NCBI GenBank
- NM_007286
- SwissProt
- Q8N3V7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGYSEEASL LRHLEKVASE EEEVPLVVYL KENAALLTAN GLHLSQNREA QQSSPAPPPA61EVHSPAADVN QNLASPSATL TTPTSNSSHN PPATDVNQNP PATVVPQSLP LSSIQQNSSE121AQLPSNGTGP ASKPSTLCAD GQPQAPAEEV RCSTLLIDKV STPATTTSTF SREATLIPSS181RPPASDFMSS SLLIDIQPNT LVVSADQEMS GRAAATTPTK VYSEVHFTLA KPPSVVNRTA241RPFGIQAPGG TSQMERSPML ERRHFGEKAP APQPPSLPDR SPRPQRHIMS RSPMVERRMM301GQRSPASERR PLGNFTAPPT YTETLSTAPL ASWVRSPPSY SVLYPSSDPK SSHLKGQAVP361ASKTGILEES MARRGSRKSM FTFVEKPKVT PNPDLLDLVQ TADEKRRQRD QGEVGVEEEP421FALGAEASNF QQEPAPRDRA SPAAAEEVVP EWASCLKSPR IQAKPKPKPN QNLSEASGKG481AELYARRQSR MEKYVIESSS HTPELARCPS PTMSLPSSWK YPTNAPGAFR VASRSPARTP541PASLYHGYLP ENGVLRPEPT KQPPYQLRPS LFVLSPIKEP AKVSPRAASP AKPSSLDLVP601NLPKGALPPS PALPRPSRSS PGLYTSPGQD SLQPTAVSPP YGGDISPVSP SRAWSPRAKQ661APRPSFSTRN AGIEAQVWKP SFCFKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.