LSBio
Human SYNPO / Synaptopodin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SYNPO / Synaptopodin Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SYNPO antibodies →
$220.00each
Catalog Number: LS-G84892-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Synaptopodin(SYNPO) (NM_001109974) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1029 | SYNPO | Synaptopodin
Origin Species
Human
NCBI GenBank
NM_007286
SwissProt
Q8N3V7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGYSEEASL LRHLEKVASE EEEVPLVVYL KENAALLTAN GLHLSQNREA QQSSPAPPPA
61EVHSPAADVN QNLASPSATL TTPTSNSSHN PPATDVNQNP PATVVPQSLP LSSIQQNSSE
121AQLPSNGTGP ASKPSTLCAD GQPQAPAEEV RCSTLLIDKV STPATTTSTF SREATLIPSS
181RPPASDFMSS SLLIDIQPNT LVVSADQEMS GRAAATTPTK VYSEVHFTLA KPPSVVNRTA
241RPFGIQAPGG TSQMERSPML ERRHFGEKAP APQPPSLPDR SPRPQRHIMS RSPMVERRMM
301GQRSPASERR PLGNFTAPPT YTETLSTAPL ASWVRSPPSY SVLYPSSDPK SSHLKGQAVP
361ASKTGILEES MARRGSRKSM FTFVEKPKVT PNPDLLDLVQ TADEKRRQRD QGEVGVEEEP
421FALGAEASNF QQEPAPRDRA SPAAAEEVVP EWASCLKSPR IQAKPKPKPN QNLSEASGKG
481AELYARRQSR MEKYVIESSS HTPELARCPS PTMSLPSSWK YPTNAPGAFR VASRSPARTP
541PASLYHGYLP ENGVLRPEPT KQPPYQLRPS LFVLSPIKEP AKVSPRAASP AKPSSLDLVP
601NLPKGALPPS PALPRPSRSS PGLYTSPGQD SLQPTAVSPP YGGDISPVSP SRAWSPRAKQ
661APRPSFSTRN AGIEAQVWKP SFCFK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

685 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.