Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SYN2 / Synapsin II Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107477-100
Catalog Number: LS-G107477
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Synapsin II(SYN2) (NM_133625), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Synapsin II | SYNII | SYNIIb | SYN2 | Synapsin-2 | SYNIIa
- Origin Species
- Human
- NCBI GenBank
- NM_003178
- SwissProt
- Q92777
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMNFLRRRLS DSSFIANLPN GYMTDLQRPE PQQPPPPPPP GPGAASASAA PPTASPGPER61RPPPASAPAP QPAPTPSVGS SFFSSLSQAV KQTAASAGLV DAPAPAPAAA RKAKVLLVVD121EPHADWAKCF RGKKVLGDYD IKVEQAEFSE LNLVAHADGT YAVDMQVLRN GTKVVRSFRP181DFVLIRQHAF GMAENEDFRH LIIGMQYAGL PSINSLESIY NFCDKPWVFA QLVAIYKTLG241GEKFPLIEQT YYPNHKEMLT LPTFPVVVKI GHAHSGMGKV KVENHYDFQD IASVVALTQT301YATAEPFIDS KYDIRVQKIG NNYKAYMRTS ISGNWKTNTG SAMLEQIAMS DRYKLWVDTC361SEMFGGLDIC AVKAVHGKDG KDYIFEVMDC SMPLIGEHQV EDRQLITELV ISKMNQLLSR421TPALSPQRPL TTQQPQSGTL KDPDSSKTPP QRPPPQGGPG QPQGMQPPGK VLPPRRLPPG481PSLPPSSSSS SSSSSSAPQR PGGPTTHGDA PSSSSSLAEA QPPLAAPPQK PQPHPQLNKS541QSLTNAFSFS ESSFFRSSAN EDEAKAETIR SLRKSFASLF SDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
582 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.