LSBio
Human SYN2 / Synapsin II Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SYN2 / Synapsin II Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SYN2 antibodies →
$153.00each
Catalog Number: LS-G81554-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Synapsin II(SYN2) (NM_133625) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Synapsin II | SYNII | SYNIIb | SYN2 | Synapsin-2 | SYNIIa
Origin Species
Human
NCBI GenBank
NM_003178
SwissProt
Q92777

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMNFLRRRLS DSSFIANLPN GYMTDLQRPE PQQPPPPPPP GPGAASASAA PPTASPGPER
61RPPPASAPAP QPAPTPSVGS SFFSSLSQAV KQTAASAGLV DAPAPAPAAA RKAKVLLVVD
121EPHADWAKCF RGKKVLGDYD IKVEQAEFSE LNLVAHADGT YAVDMQVLRN GTKVVRSFRP
181DFVLIRQHAF GMAENEDFRH LIIGMQYAGL PSINSLESIY NFCDKPWVFA QLVAIYKTLG
241GEKFPLIEQT YYPNHKEMLT LPTFPVVVKI GHAHSGMGKV KVENHYDFQD IASVVALTQT
301YATAEPFIDS KYDIRVQKIG NNYKAYMRTS ISGNWKTNTG SAMLEQIAMS DRYKLWVDTC
361SEMFGGLDIC AVKAVHGKDG KDYIFEVMDC SMPLIGEHQV EDRQLITELV ISKMNQLLSR
421TPALSPQRPL TTQQPQSGTL KDPDSSKTPP QRPPPQGGPG QPQGMQPPGK VLPPRRLPPG
481PSLPPSSSSS SSSSSSAPQR PGGPTTHGDA PSSSSSLAEA QPPLAAPPQK PQPHPQLNKS
541QSLTNAFSFS ESSFFRSSAN EDEAKAETIR SLRKSFASLF SD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.