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SYDE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SYDE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108984-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SYDE2 (NM_032184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHDLPPDSGA RRGGRGLADH SFPAGARAPG QPPSRGAAYR RACPRDGERG GGGRPRQQVS
61PPRSPQREPR GGQLRTPRMR PSCSRSLESL RVGAKPPPFQ RWPSDSWIRC GAHRDWDEPP
121PRGGRMDGWS GDRARAAAPT GLQPPGCKDH GCSSGSPFRD PAGSSVIRSG KGDRQEGPSF
181LRPPAVTVKK LQKWMYKGRL LSLGMKGRAR GTAPKVTGTQ AASPNVGALK VRENRVLSVP
241PDQRITLTDL FENAYGSSMK GRELEELKDN IEFRGHKPLN SITVSKKRNW LYQSTLRPLN
301LEEENKKCQD RSHLSISPVS LPKHQLSQSF LKSSKEYCTY VVCNATNSSL SKNCALDFNE
361ENDADDEGEI WYNPIPEDDD LGISSALSFG EADSAVLKLP AVNLSMLSGS DLMKAERHTE
421DSLCSSEHAG DIQTTRSNGM NPIHPAHSTE FVQQYKQKLG HKTQEGIMVE DSPMLKSPFA
481GSGILAATNS TELGIMEPSS PNPSPVKKGS SINWSLPDKI KSPRTVRKLS MKMKKLPEFS
541RKLSVKGTLN YINSPDNTPS LSKYNCREVH HTDILPSGNT TTAAKRNVIS RYHLDTSVSS
601QQSYQKKNSM SSKYSCKGGY LSDGDSPELT TKASKHGSEN KFGKGKEIIS NSCSKNEIDI
661DAFRHYSFSD QPKCSQYISG LMSVHFYGAE DLKPPRIDSK DVFCAIQVDS VNKARTALLT
721CRTTFLDMDH TFNIEIENAQ HLKLVVFSWE PTPRKNRVCC HGTVVLPTLF RVTKTHQLAV
781KLEPRGLIYV KVTLMEQWEN SLHGLDINQE PIIFGVDIQK VVEKENIGLM VPLLIQKCIM
841EIEKRGCQVV GLYRLCGSAA VKKELREAFE RDSKAVGLCE NQYPDINVIT GVLKDYLREL
901PSPLITKQLY EAVLDAMAKS PLKMSSNGCE NDPGDSKYTV DLLDCLPEIE KATLKMLLDH
961LKLVASYHEV NKMTCQNLAV CFGPVLLSQR QEPSTHNNRV FTDSEELASA LDFKKHIEVL
1021HYLLQLWPVQ RLTVKKSTDN LFPEQKSSLN YLRQKKERPH MLNLSGTDSS GVLRPRQNRL
1081DSPLSNRYAG DWSSCGENYF LNTKENLNDV DYDDVPSEDR KIGENYSKMD GPEVMIEQPI
1141PMSKECTFQT YLTMQTVEST VDRKNNLKDL QESIDTLIGN LERELNKNKL NMSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1194 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.