SYDE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G108984-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SYDE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96462-20
Catalog Number: LS-G96462
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SYDE2 (NM_032184) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHDLPPDSGA RRGGRGLADH SFPAGARAPG QPPSRGAAYR RACPRDGERG GGGRPRQQVS61PPRSPQREPR GGQLRTPRMR PSCSRSLESL RVGAKPPPFQ RWPSDSWIRC GAHRDWDEPP121PRGGRMDGWS GDRARAAAPT GLQPPGCKDH GCSSGSPFRD PAGSSVIRSG KGDRQEGPSF181LRPPAVTVKK LQKWMYKGRL LSLGMKGRAR GTAPKVTGTQ AASPNVGALK VRENRVLSVP241PDQRITLTDL FENAYGSSMK GRELEELKDN IEFRGHKPLN SITVSKKRNW LYQSTLRPLN301LEEENKKCQD RSHLSISPVS LPKHQLSQSF LKSSKEYCTY VVCNATNSSL SKNCALDFNE361ENDADDEGEI WYNPIPEDDD LGISSALSFG EADSAVLKLP AVNLSMLSGS DLMKAERHTE421DSLCSSEHAG DIQTTRSNGM NPIHPAHSTE FVQQYKQKLG HKTQEGIMVE DSPMLKSPFA481GSGILAATNS TELGIMEPSS PNPSPVKKGS SINWSLPDKI KSPRTVRKLS MKMKKLPEFS541RKLSVKGTLN YINSPDNTPS LSKYNCREVH HTDILPSGNT TTAAKRNVIS RYHLDTSVSS601QQSYQKKNSM SSKYSCKGGY LSDGDSPELT TKASKHGSEN KFGKGKEIIS NSCSKNEIDI661DAFRHYSFSD QPKCSQYISG LMSVHFYGAE DLKPPRIDSK DVFCAIQVDS VNKARTALLT721CRTTFLDMDH TFNIEIENAQ HLKLVVFSWE PTPRKNRVCC HGTVVLPTLF RVTKTHQLAV781KLEPRGLIYV KVTLMEQWEN SLHGLDINQE PIIFGVDIQK VVEKENIGLM VPLLIQKCIM841EIEKRGCQVV GLYRLCGSAA VKKELREAFE RDSKAVGLCE NQYPDINVIT GVLKDYLREL901PSPLITKQLY EAVLDAMAKS PLKMSSNGCE NDPGDSKYTV DLLDCLPEIE KATLKMLLDH961LKLVASYHEV NKMTCQNLAV CFGPVLLSQR QEPSTHNNRV FTDSEELASA LDFKKHIEVL1021HYLLQLWPVQ RLTVKKSTDN LFPEQKSSLN YLRQKKERPH MLNLSGTDSS GVLRPRQNRL1081DSPLSNRYAG DWSSCGENYF LNTKENLNDV DYDDVPSEDR KIGENYSKMD GPEVMIEQPI1141PMSKECTFQT YLTMQTVEST VDRKNNLKDL QESIDTLIGN LERELNKNKL NMSFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1194 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
