Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SVIL / Supervillin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117518-100
Catalog Number: LS-G117518
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Supervillin(SVIL) (NM_003174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Archvillin | p205/p250 | Supervillin | SVIL
- Origin Species
- Human
- NCBI GenBank
- NM_003174
- SwissProt
- O95425
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 200.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRKERIARR LEGIENDTQP ILLQSCTGLV THRLLEEDTP RYMRASDPAS PHIGRSNEEE61ETSDSSLEKQ TRSKYCTETS GVHGDSPYGS GTMDTHSLES KAERIARYKA ERRRQLAEKY121GLTLDPEADS EYLSRYTKSR KEPDAVEKRG GKSDKQEESS RDASSLYPGT ETMGLRTCAG181ESKDYALHVG DGSSDPEVLL NIENQRRGQE LSATRQAHDL SPAAESSSTF SFSGRDSSFT241EVPRSPKHAH SSSLQQAASR SPSFGDPQLS PEARPRCTSH SETPTVDDEE KVDERAKLSV301AAKRLLFREM EKSFDEQNVP KRRSRNTAVE QRLRRLQDRS LTQPITTEEV VIAATLQASA361HQKALAKDQT NEGKELAEQG EPDSSTLSLA EKLALFNKLS QPVSKAISTR NRIDTRQRRM421NARYQTQPVT LGEVEQVQSG KLIPFSPAVN TSVSTVASTV APMYAGDLRT KPPLDHNASA481TDYKFSSSIE NSDSPVRSIL KSQAWQPLVE GSENKGMLRE YGETESKRAL TGRDSGMEKY541GSFEEAEASY PILNRAREGD SHKESKYAVP RRGSLERANP PITHLGDEPK EFSMAKMNAQ601GNLDLRDRLP FEEKVEVENV MKRKFSLRAA EFGEPTSEQT GTAAGKTIAQ TTAPVSWKPQ661DSSEQPQEKL CKNPCAMFAA GEIKTPTGEG LLDSPSKTMS IKERLALLKK SGEEDWRNRL721SRRQEGGKAP ASSLHTQEAG RSLIKKRVTE SRESQMTIEE RKQLITVREE AWKTRGRGAA781NDSTQFTVAG RMVKKGLASP TAITPVASAI CGKTRGTTPV SKPLEDIEAR PDMQLESDLK841LDRLETFLRR LNNKVGGMHE TVLTVTGKSV KEVMKPDDDE TFAKFYRSVD YNMPRSPVEM901DEDFDVIFDP YAPKLTSSVA EHKRAVRPKR RVQASKNPLK MLAAREDLLQ EYTEQRLNVA961FMESKRMKVE KMSSNSNFSE VTLAGLASKE NFSNVSLRSV NLTEQNSNNS AVPYKRLMLL1021QIKGRRHVQT RLVEPRASAL NSGDCFLLLS PHCCFLWVGE FANVIEKAKA SELATLIQTK1081RELGCRATYI QTIEEGINTH THAAKDFWKL LGGQTSYQSA GDPKEDELYE AAIIETNCIY1141RLMDDKLVPD DDYWGKIPKC SLLQPKEVLV FDFGSEVYVW HGKEVTLAQR KIAFQLAKHL1201WNGTFDYENC DINPLDPGEC NPLIPRKGQG RPDWAIFGRL TEHNETILFK EKFLDWTELK1261RSNEKNPGEL AQHKEDPRTD VKAYDVTRMV SMPQTTAGTI LDGVNVGRGY GLVEGHDRRQ1321FEITSVSVDV WHILEFDYSR LPKQSIGQFH EGDAYVVKWK FMVSTAVGSR QKGEHSVRAA1381GKEKCVYFFW QGRHSTVSEK GTSALMTVEL DEERGAQVQV LQGKEPPCFL QCFQGGMVVH1441SGRREEEEEN VQSEWRLYCV RGEVPVEGNL LEVACHCSSL RSRTSMVVLN VNKALIYLWH1501GCKAQAHTKE VGRTAANKIK EQCPLEAGLH SSSKVTIHEC DEGSEPLGFW DALGRRDRKA1561YDCMLQDPGS FNFAPRLFIL SSSSGDFAAT EFVYPARAPS VVSSMPFLQE DLYSAPQPAL1621FLVDNHHEVY LWQGWWPIEN KITGSARIRW ASDRKSAMET VLQYCKEKNL KKPAPKSYLI1681HAGLEPLTFT NMFPSWEHRE DIAEITEMDT EVSNQITLVE DVLAKLCKTI YPLADLLARP1741LPEGVDPLKL EIYLTDEDFE FALDMTRDEY NALPAWKQVN LKKAKGLFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1788 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.