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Human SVIL / Supervillin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SVIL / Supervillin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SVIL antibodies →
$220.00each
Catalog Number: LS-G80465-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Supervillin(SVIL) (NM_003174) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Archvillin | p205/p250 | Supervillin | SVIL
Origin Species
Human
NCBI GenBank
NM_003174
SwissProt
O95425

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
200.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MKRKERIARR LEGIENDTQP ILLQSCTGLV THRLLEEDTP RYMRASDPAS PHIGRSNEEE
61ETSDSSLEKQ TRSKYCTETS GVHGDSPYGS GTMDTHSLES KAERIARYKA ERRRQLAEKY
121GLTLDPEADS EYLSRYTKSR KEPDAVEKRG GKSDKQEESS RDASSLYPGT ETMGLRTCAG
181ESKDYALHVG DGSSDPEVLL NIENQRRGQE LSATRQAHDL SPAAESSSTF SFSGRDSSFT
241EVPRSPKHAH SSSLQQAASR SPSFGDPQLS PEARPRCTSH SETPTVDDEE KVDERAKLSV
301AAKRLLFREM EKSFDEQNVP KRRSRNTAVE QRLRRLQDRS LTQPITTEEV VIAATLQASA
361HQKALAKDQT NEGKELAEQG EPDSSTLSLA EKLALFNKLS QPVSKAISTR NRIDTRQRRM
421NARYQTQPVT LGEVEQVQSG KLIPFSPAVN TSVSTVASTV APMYAGDLRT KPPLDHNASA
481TDYKFSSSIE NSDSPVRSIL KSQAWQPLVE GSENKGMLRE YGETESKRAL TGRDSGMEKY
541GSFEEAEASY PILNRAREGD SHKESKYAVP RRGSLERANP PITHLGDEPK EFSMAKMNAQ
601GNLDLRDRLP FEEKVEVENV MKRKFSLRAA EFGEPTSEQT GTAAGKTIAQ TTAPVSWKPQ
661DSSEQPQEKL CKNPCAMFAA GEIKTPTGEG LLDSPSKTMS IKERLALLKK SGEEDWRNRL
721SRRQEGGKAP ASSLHTQEAG RSLIKKRVTE SRESQMTIEE RKQLITVREE AWKTRGRGAA
781NDSTQFTVAG RMVKKGLASP TAITPVASAI CGKTRGTTPV SKPLEDIEAR PDMQLESDLK
841LDRLETFLRR LNNKVGGMHE TVLTVTGKSV KEVMKPDDDE TFAKFYRSVD YNMPRSPVEM
901DEDFDVIFDP YAPKLTSSVA EHKRAVRPKR RVQASKNPLK MLAAREDLLQ EYTEQRLNVA
961FMESKRMKVE KMSSNSNFSE VTLAGLASKE NFSNVSLRSV NLTEQNSNNS AVPYKRLMLL
1021QIKGRRHVQT RLVEPRASAL NSGDCFLLLS PHCCFLWVGE FANVIEKAKA SELATLIQTK
1081RELGCRATYI QTIEEGINTH THAAKDFWKL LGGQTSYQSA GDPKEDELYE AAIIETNCIY
1141RLMDDKLVPD DDYWGKIPKC SLLQPKEVLV FDFGSEVYVW HGKEVTLAQR KIAFQLAKHL
1201WNGTFDYENC DINPLDPGEC NPLIPRKGQG RPDWAIFGRL TEHNETILFK EKFLDWTELK
1261RSNEKNPGEL AQHKEDPRTD VKAYDVTRMV SMPQTTAGTI LDGVNVGRGY GLVEGHDRRQ
1321FEITSVSVDV WHILEFDYSR LPKQSIGQFH EGDAYVVKWK FMVSTAVGSR QKGEHSVRAA
1381GKEKCVYFFW QGRHSTVSEK GTSALMTVEL DEERGAQVQV LQGKEPPCFL QCFQGGMVVH
1441SGRREEEEEN VQSEWRLYCV RGEVPVEGNL LEVACHCSSL RSRTSMVVLN VNKALIYLWH
1501GCKAQAHTKE VGRTAANKIK EQCPLEAGLH SSSKVTIHEC DEGSEPLGFW DALGRRDRKA
1561YDCMLQDPGS FNFAPRLFIL SSSSGDFAAT EFVYPARAPS VVSSMPFLQE DLYSAPQPAL
1621FLVDNHHEVY LWQGWWPIEN KITGSARIRW ASDRKSAMET VLQYCKEKNL KKPAPKSYLI
1681HAGLEPLTFT NMFPSWEHRE DIAEITEMDT EVSNQITLVE DVLAKLCKTI YPLADLLARP
1741LPEGVDPLKL EIYLTDEDFE FALDMTRDEY NALPAWKQVN LKKAKGLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1788 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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