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SUN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SUN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130156-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUN1 (NM_001171944), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0810 | Protein unc-84 homolog A | UNC84A | Sad1/unc-84 protein-like 1 | Unc-84 homolog A | Unc-84 homolog A (C. elegans) | Sad1 unc-84 domain protein 1 | SUN1
Origin Species
Human
NCBI GenBank
AF202724
SwissProt
O94901

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFSRLHMYS PPQCVPENTG YTYALSSSYS SDALDFETEH KLDPVFDSPR MSRRSLRLAT
61TACTLGDGEA VGADSGTSSA VSLKNRAART TKQRRSTNKS AFSINHVSRQ VTSSGVSHGG
121TVSLQDAVTR RPPVLDESWI REQTTVDHFW GKAASGVFWW LGIGWYQFVT LISWLNVFLL
181TRCLRNICKF LVLLIPLFLL LGLSLRGQGN FFSFLPVLNW ASMHRTQRVD DPQDVFKPTT
241SRLKQPLQGD SEAFPWHWMS GVEQQVASLS GQCHHHGENL RELTTLLQKL QARVDQMEGG
301AAGPSASVRD AVGQPPRETD FMAFHQEHEV RMSHLEDILG KLREKSEAIQ KELEQTKQKT
361ISAVGEQLLP TVEHLQLELD QLKSELSSWR HVKTGCETVD AVQERVDVQV REMVKLLFSE
421DQQGGSLEQL LQRFSSQFVS KGDLQTMLRD LQLQILRNVT HHVSVTKQLP TSEAVVSAVS
481EAGASGITEA QARAIVNSAL KLYSQDKTGM VDFALESGGG SILSTRCSET YETKTALMSL
541FGIPLWYFSQ SPRVVIQPDI YPGNCWAFKG SQGYLVVRLS MMIHPAAFTL EHIPKTLSPT
601GNISSAPKDF AVYGLENEYQ EEGQLLGQFT YDQDGESLQM FQALKRPDDT AFQIVELRIF
661SNWGHPEYTC LYRFRVHGEP VK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

682 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.