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SUN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SUN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G125172-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUN1 (NM_001130965), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0810 | Protein unc-84 homolog A | UNC84A | Sad1/unc-84 protein-like 1 | Unc-84 homolog A | Unc-84 homolog A (C. elegans) | Sad1 unc-84 domain protein 1 | SUN1
Origin Species
Human
NCBI GenBank
AF202724
SwissProt
O94901

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFSRLHMYS PPQCVPENTG YTYALSSSYS SDALDFETEH KLDPVFDSPR MSRRSLRLAT
61TACTLGDGEA VGADSGTSSA VSLKNRAART TKQRRSTNKS AFSINHVSRQ VTSSGVSHGG
121TVSLQDAVTR RPPVLDESWI REQTTVDHFW GLDDDGDLKG GNKAAIQGNG DVGAAAATAH
181NGFSCSNCSM LSERKDVLTA HPAAPGPVSR VYSRDRNQKC YFLLQILRRI GAVGQAVSRT
241AWSALWLAVV APGKAASGVF WWLGIGWYQF VTLISWLNVF LLTRCLRNIC KFLVLLIPLF
301LLLAGLSLRG QGNFFSFLPV LNWASMHRTQ RVDDPQDVFK PTTSRLKQPL QGDSEAFPWH
361WMSGVEQQVA SLSGQCHHHG ENLRELTTLL QKLQARVDQM EGGAAGPSAS VRDAVGQPPR
421ETDFMAFHQE HEVRMSHLED ILGKLREKSE AIQKELEQTK QKTISAVGEQ LLPTVEHLQL
481ELDQLKSELS SWRHVKTGCE TVDAVQERVD VQVREMVKLL FSEDQQGGSL EQLLQRFSSQ
541FVSKGDLQTM LRDLQLQILR NVTHHVSVTK QLPTSEAVVS AVSEAGASGI TEAQARAIVN
601SALKLYSQDK TGMVDFALES GGGSILSTRC SETYETKTAL MSLFGIPLWY FSQSPRVVIQ
661PDIYPGNCWA FKGSQGYLVV RLSMMIHPAA FTLEHIPKTL SPTGNISSAP KDFAVYGLEN
721EYQEEGQLLG QFTYDQDGES LQMFQALKRP DDTAFQIVEL RIFSNWGHPE YTCLYRFRVH
781GEPVK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

785 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Lysate samples are stable for 12 months when stored at -80°C.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.