TIA-1 Antibody (clone TIA-1)
Catalog Number: LS-C312188-0.1
0.1 mL / $355.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sulfatase 1 / SULF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113429-100
Catalog Number: LS-G113429
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sulfatase 1(SULF1) (NM_015170), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Extracellular sulfatase Sulf-1 | HSULF-1 | KIAA1077 | SULF1 | Sulfatase FP | SULF-1 | Sulfatase 1
- Origin Species
- Human
- NCBI GenBank
- AB029000
- SwissProt
- Q8IWU6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKYSCCALVL AVLGTELLGS LCSTVRSPRF RGRIQQERKN IRPNIILVLT DDQDVELGSL61QVMNKTRKIM EHGGATFINA FVTTPMCCPS RSSMLTGKYV HNHNVYTNNE NCSSPSWQAM121HEPRTFAVYL NNTGYRTAFF GKYLNEYNGS YIPPGWREWL GLIKNSRFYN YTVCRNGIKE181KHGFDYAKDY FTDLITNESI NYFKMSKRMY PHRPVMMVIS HAAPHGPEDS APQFSKLYPN241ASQHITPSYN YAPNMDKHWI MQYTGPMLPI HMEFTNILQR KRLQTLMSVD DSVERLYNML301VETGELENTY IIYTADHGYH IGQFGLVKGK SMPYDFDIRV PFFIRGPSVE PGSIVPQIVL361NIDLAPTILD IAGLDTPPDV DGKSVLKLLD PEKPGNRFRT NKKAKIWRDT FLVERGKFLR421KKEESSKNIQ QSNHLPKYER VKELCQQARY QTACEQPGQK WQCIEDTSGK LRIHKCKGPS481DLLTVRQSTR NLYARGFHDK DKECSCRESG YRASRSQRKS QRQFLRNQGT PKYKPRFVHT541RQTRSLSVEF EGEIYDINLE EEEELQVLQP RNIAKRHDEG HKGPRDLQAS SGGNRGRMLA601DSSNAVGPPT TVRVTHKCFI LPNDSIHCER ELYQSARAWK DHKAYIDKEI EALQDKIKNL661REVRGHLKRR KPEECSCSKQ SYYNKEKGVK KQEKLKSHLH PFKEAAQEVD SKLQLFKENN721RRRKKERKEK RRQRKGEECS LPGLTCFTHD NNHWQTAPFW NLGSFCACTS SNNNTYWCLR781TVNETHNFLF CEFATGFLEY FDMNTDPYQL TNTVHTVERG ILNQLHVQLM ELRSCQGYKQ841CNPRPKNLDV GNKDGGSYDL HRGQLWDGWE GTRTRPLEQKLISEEDLAANDILDYKDDDDKV
871 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
