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Human Sulfatase 1 / SULF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human Sulfatase 1 / SULF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SULF1 antibodies →
$220.00each
Catalog Number: LS-G83895-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Sulfatase 1(SULF1) (NM_015170) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Extracellular sulfatase Sulf-1 | HSULF-1 | KIAA1077 | SULF1 | Sulfatase FP | SULF-1 | Sulfatase 1
Origin Species
Human
NCBI GenBank
AB029000
SwissProt
Q8IWU6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKYSCCALVL AVLGTELLGS LCSTVRSPRF RGRIQQERKN IRPNIILVLT DDQDVELGSL
61QVMNKTRKIM EHGGATFINA FVTTPMCCPS RSSMLTGKYV HNHNVYTNNE NCSSPSWQAM
121HEPRTFAVYL NNTGYRTAFF GKYLNEYNGS YIPPGWREWL GLIKNSRFYN YTVCRNGIKE
181KHGFDYAKDY FTDLITNESI NYFKMSKRMY PHRPVMMVIS HAAPHGPEDS APQFSKLYPN
241ASQHITPSYN YAPNMDKHWI MQYTGPMLPI HMEFTNILQR KRLQTLMSVD DSVERLYNML
301VETGELENTY IIYTADHGYH IGQFGLVKGK SMPYDFDIRV PFFIRGPSVE PGSIVPQIVL
361NIDLAPTILD IAGLDTPPDV DGKSVLKLLD PEKPGNRFRT NKKAKIWRDT FLVERGKFLR
421KKEESSKNIQ QSNHLPKYER VKELCQQARY QTACEQPGQK WQCIEDTSGK LRIHKCKGPS
481DLLTVRQSTR NLYARGFHDK DKECSCRESG YRASRSQRKS QRQFLRNQGT PKYKPRFVHT
541RQTRSLSVEF EGEIYDINLE EEEELQVLQP RNIAKRHDEG HKGPRDLQAS SGGNRGRMLA
601DSSNAVGPPT TVRVTHKCFI LPNDSIHCER ELYQSARAWK DHKAYIDKEI EALQDKIKNL
661REVRGHLKRR KPEECSCSKQ SYYNKEKGVK KQEKLKSHLH PFKEAAQEVD SKLQLFKENN
721RRRKKERKEK RRQRKGEECS LPGLTCFTHD NNHWQTAPFW NLGSFCACTS SNNNTYWCLR
781TVNETHNFLF CEFATGFLEY FDMNTDPYQL TNTVHTVERG ILNQLHVQLM ELRSCQGYKQ
841CNPRPKNLDV GNKDGGSYDL HRGQLWDGWE G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

871 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.