Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUGT1 / SGT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125147-100
Catalog Number: LS-G125147
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUGT1 (NM_001130912), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein 40-6-3 | SGT1 | SGT1B protein | SUGT1 | Putative 40-6-3 protein
- Origin Species
- Human
- NCBI GenBank
- NM_006704
- SwissProt
- Q9Y2Z0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAGTAT SQRFFQSFSD ALIDEDPQAA LEELTKALEQ KPDDAQYYCQ RAYCHILLGN61YCVAVADAKK SLELNPNNST AMLRKGICEY HEKNYAAALE TFTEGQKLDI ETGFHRVGQA121GLQLLTSSDP PALDSQSAGI TGADANFSVW IKRCQEAQNG SESEVWTHQS KIKYDWYQTE181SQVVITLMIK NVQKNDVNVE FSEKELSALV KLPSGEDYNL KLELLHPIIP EQSTFKVLST241KIEIKLKKPE AVRWEKLEGQ GDVPTPKQFV ADVKNLYPSS SPYTRNWDKL VGEIKEEEKN301EKLEGDAALN RLFQQIYSDG SDEVKRAMNK SFMESGGTVL STNWSDVGKR KVEINPPDDM361EWKKYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
365 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.