Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SUGT1 / SGT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115139-100
Catalog Number: LS-G115139
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SUGT1 (NM_006704), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein 40-6-3 | SGT1 | SGT1B protein | SUGT1 | Putative 40-6-3 protein
- Origin Species
- Human
- NCBI GenBank
- NM_006704
- SwissProt
- Q9Y2Z0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAAGTAT SQRFFQSFSD ALIDEDPQAA LEELTKALEQ KPDDAQYYCQ RAYCHILLGN61YCVAVADAKK SLELNPNNST AMLRKGICEY HEKNYAAALE TFTEGQKLDS ADANFSVWIK121RCQEAQNGSE SEVWTHQSKI KYDWYQTESQ VVITLMIKNV QKNDVNVEFS EKELSALVKL181PSGEDYNLKL ELLHPIIPEQ STFKVLSTKI EIKLKKPEAV RWEKLEGQGD VPTPKQFVAD241VKNLYPSSSP YTRNWDKLVG EIKEEEKNEK LEGDAALNRL FQQIYSDGSD EVKRAMNKSF301MESGGTVLST NWSDVGKRKV EINPPDDMEW KKYTRTRPLE QKLISEEDLA ANDILDYKDD361DDKV
364 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.