LSBio
STXBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

STXBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all STXBP3 antibodies →
$504.00each
Catalog Number: LS-G114754-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Munc18c(STXBP3) (NM_007269), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MUNC18-3 | Platelet Sec1 protein | Protein unc-18 homolog C | Syntaxin 4 binding protein | MUNC18C | UNC-18C | Unc18-3 | Protein unc-18 homolog 3 | PSP | Syntaxin binding protein 3 | Syntaxin-binding protein 3 | STXBP3
Origin Species
Human
NCBI GenBank
NM_007269
SwissProt
O00186

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPVAERGL KSVVWQKIKA TVFDDCKKEG EWKIMLLDEF TTKLLASCCK MTDLLEEGIT
61VVENIYKNRE PVRQMKALYF ITPTSKSVDC FLHDFASKSE NKYKAAYIYF TDFCPDNLFN
121KIKASCSKSI RRCKEINISF IPHESQVYTL DVPDAFYYCY SPDPGNAKGK DAIMETMADQ
181IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKSLIKGK THSQLLIIDR
241GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA ILEEEDDLWV RIRHRHIAVV
301LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ITKQVVHLNL AEDCMNKFKL
361NIEKLCKTEQ DLALGTDAEG QKVKDSMRVL LPVLLNKNHD NCDKIRAILL YIFSINGTTE
421ENLDRLIQNV KIENESDMIR NWSYLGVPIV PQSQQGKPLR KDRSAEETFQ LSRWTPFIKD
481IMEDAIDNRL DSKEWPYCSQ CPAVWNGSGA VSARQKPRAN YLEDRKNGSK LIVFVIGGIT
541YSEVRCAYEV SQAHKSCEVI IGSTHVLTPK KLLDDIKMLN KPKDKVSLIK DE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.