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Human STXBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human STXBP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all STXBP3 antibodies →
$153.00each
Catalog Number: LS-G79031-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Munc18c(STXBP3) (NM_007269) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MUNC18-3 | Platelet Sec1 protein | Protein unc-18 homolog C | Syntaxin 4 binding protein | MUNC18C | UNC-18C | Unc18-3 | Protein unc-18 homolog 3 | PSP | Syntaxin binding protein 3 | Syntaxin-binding protein 3 | STXBP3
Origin Species
Human
NCBI GenBank
NM_007269
SwissProt
O00186

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPVAERGL KSVVWQKIKA TVFDDCKKEG EWKIMLLDEF TTKLLASCCK MTDLLEEGIT
61VVENIYKNRE PVRQMKALYF ITPTSKSVDC FLHDFASKSE NKYKAAYIYF TDFCPDNLFN
121KIKASCSKSI RRCKEINISF IPHESQVYTL DVPDAFYYCY SPDPGNAKGK DAIMETMADQ
181IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKSLIKGK THSQLLIIDR
241GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA ILEEEDDLWV RIRHRHIAVV
301LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ITKQVVHLNL AEDCMNKFKL
361NIEKLCKTEQ DLALGTDAEG QKVKDSMRVL LPVLLNKNHD NCDKIRAILL YIFSINGTTE
421ENLDRLIQNV KIENESDMIR NWSYLGVPIV PQSQQGKPLR KDRSAEETFQ LSRWTPFIKD
481IMEDAIDNRL DSKEWPYCSQ CPAVWNGSGA VSARQKPRAN YLEDRKNGSK LIVFVIGGIT
541YSEVRCAYEV SQAHKSCEVI IGSTHVLTPK KLLDDIKMLN KPKDKVSLIK DE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.