Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SRPK3 / MSSK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113999-100
Catalog Number: LS-G113999
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STK23(SRPK3) (NM_014370), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MSSK1 | Serine/threonine kinase 23 | SFRS protein kinase 3 | SR-protein-specific kinase 3 | SRPK3 | STK23 | SRSF protein kinase 3 | MSSK-1
- Origin Species
- Human
- NCBI GenBank
- NM_014370
- SwissProt
- Q9UPE1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSASTGGGGD SGGSGGSSSS SQASCGPESS GSELALATPV PQMLQGLLGS DDEEQEDPKD61YCKGGYHPVK IGDVFNGRYH VVRKLGWGHF STVWLCWDIQ RKRFVALKVV KSAGHYTETA121VDEIKLLKCV RDSDPSDPKR ETIVQLIDDF RISGVNGVHV CMVLEVLGHQ LLKWIIKSNY181QGLPVPCVKS IVRQVLHGLD YLHTKCKIIH TDIKPENILL CVGDAYIRRL AAEATEWQQA241GAPPPSRSIV STAPQEVLTG KLSKNKRKKM RRKRKQQKRL LEERLRDLQR LEAMEAATQA301EDSGLRLDGG SGSTSSSGCH PGGARAGPSP ASSSPAPGGG RSLSAGSQTS GFSGSLFSPA361SCSILSGSSN QRETGGLLSP STPFGASNLL VNPLEPQNAD KIKIKIADLG NACWVHKHFT421EDIQTRQYRA VEVLIGAEYG PPADIWSTAC MAFELATGDY LFEPHSGEDY SRDEDHIAHI481VELLGDIPPA FALSGRYSRE FFNRRGELRH IHNLKHWGLY EVLMEKYEWP LEQATQFSAF541LLPMMEYIPE KRASAADCLQ HPWLNPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
566 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.