LSBio
Human SRPK3 / MSSK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SRPK3 / MSSK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G86861-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human STK23(SRPK3) (NM_014370) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MSSK1 | Serine/threonine kinase 23 | SFRS protein kinase 3 | SR-protein-specific kinase 3 | SRPK3 | STK23 | SRSF protein kinase 3 | MSSK-1
Origin Species
Human
NCBI GenBank
NM_014370
SwissProt
Q9UPE1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSASTGGGGD SGGSGGSSSS SQASCGPESS GSELALATPV PQMLQGLLGS DDEEQEDPKD
61YCKGGYHPVK IGDVFNGRYH VVRKLGWGHF STVWLCWDIQ RKRFVALKVV KSAGHYTETA
121VDEIKLLKCV RDSDPSDPKR ETIVQLIDDF RISGVNGVHV CMVLEVLGHQ LLKWIIKSNY
181QGLPVPCVKS IVRQVLHGLD YLHTKCKIIH TDIKPENILL CVGDAYIRRL AAEATEWQQA
241GAPPPSRSIV STAPQEVLTG KLSKNKRKKM RRKRKQQKRL LEERLRDLQR LEAMEAATQA
301EDSGLRLDGG SGSTSSSGCH PGGARAGPSP ASSSPAPGGG RSLSAGSQTS GFSGSLFSPA
361SCSILSGSSN QRETGGLLSP STPFGASNLL VNPLEPQNAD KIKIKIADLG NACWVHKHFT
421EDIQTRQYRA VEVLIGAEYG PPADIWSTAC MAFELATGDY LFEPHSGEDY SRDEDHIAHI
481VELLGDIPPA FALSGRYSRE FFNRRGELRH IHNLKHWGLY EVLMEKYEWP LEQATQFSAF
541LLPMMEYIPE KRASAADCLQ HPWLNP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

566 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.