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SPIRE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPIRE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPIRE1 antibodies →
$725.00each
Catalog Number: LS-G111234-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPIRE1 (NM_020148), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1135 | Spir-1 | Spire homolog 1 (Drosophila) | SPIR1 | SPIRE1
Origin Species
Human
SwissProt
Q08AE8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MANTVEADGS NDEGYEAAEE GLGDEDEKRK ISAIRSYRDV MKLCAAHLPT ESDAPNHYQA
61VCRALFAETM ELHTFLTKIK SAKENLKKIQ EMEKSDESST DLEELKNADW ARFWVQVMRD
121LRNGVKLKKV QERQYNPLPI EYQLTPYEML MDDIRCKRYT LRKVMVNGDI PPRLKKSAHE
181IILDFIRSRP PLNPVSARKL KPTPPRPRSL HERILEEIKA ERKLRPVSPE EIRRSRLDVT
241TPESTKNLVE SSMVNGGLTS QTKENGLSTS QQVPAQRKKL LRAPTLAELD SSESEEETLH
301KSTSSSSVSP SFPEEPVLEA VSTRKKPPKF LPISSTPQPE RRQPPQRRHS IEKETPTNVR
361QFLPPSRQSS RSLEEFCYPV ECLALTVEEV MHIRQVLVKA ELEKYQQYKD IYTALKKGKL
421CFCCRTRRFS FFTWSYTCQF CKRPVCSQCC KKMRLPSKPY STLPIFSLGP SALQRGESSM
481RSEKPSTAHH RPLRSIARFS SKSKSMDKSD EELQFPKELM EDWSTMEVCV DCKKFISEII
541SSSRRSLVLA NKRARLKRKT QSFYMSSPGP SEYCPSERTI SEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

583 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.