SPIRE1 Antibody
Catalog Number: LS-C832058
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SPIRE1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G94141-20
Catalog Number: LS-G94141
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPIRE1 (NM_001128627) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1135 | Spir-1 | Spire homolog 1 (Drosophila) | SPIR1 | SPIRE1
- Origin Species
- Human
- SwissProt
- Q08AE8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METEVIESLG IIIYKALDYG LKENEERELS PPLEQLIDHM ANTVEADGSN DEGYEAAEEG61LGDEDEKRKI SAIRSYRDVM KLCAAHLPTE SDAPNHYQAV CRALFAETME LHTFLTKIKS121AKENLKKIQE MEKSDESSTD LEELKNADWA RFWVQVMRDL RNGVKLKKVQ ERQYNPLPIE181YQLTPYEMLM DDIRCKRYTL RKVMVNGDIP PRLKKSAHEI ILDFIRSRPP LNPVSARKLK241PTPPRPRSLH ERILEEIKAE RKLRPVSPEE IRRSRLDVTT PESTKNLVES SMVNGGLTSQ301TKENGLSTSQ QVPAQRKKLL RAPTLAELDS SESEEETLHK STSSSSVSPS FPEEPVLEAV361STRKKPPKFL PISSTPQPER RQPPQRRHSI EKETPTNVRQ FLPPSRQSSR SLEEFCYPVE421CLALTVEEVM HIRQVLVKAE LEKYQQYKDI YTALKKGKLC FCCRTRRFSF FTWSYTCQFC481KRPVCSQCCK KMRLPSKPYS TLPIFSLGPS ALQRGESSMR SEKPSTAHHR PLRSIARFSS541KSKSMDKSDE ELQFPKELME DWSTMEVCVD CKKFISEIIS SSRRSLVLAN KRARLKRKTQ601SFYMSSPGPS EYCPSERTIS EITRTRPLEQKLISEEDLAANDILDYKDDDDKV
622 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
