Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPIDR / KIAA0146 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120182-100
Catalog Number: LS-G120182
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0146(SPIDR) (NM_001080394), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0146
- Origin Species
- Human
- NCBI GenBank
- D63480
- SwissProt
- Q14159
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRGSRARGS KRKRSWNTEC PSFPGERPLQ VRRAGLRTAG AAASLSEAWL RCGEGFQNTS61GNPSLTAEEK TITEKHLELC PRPKQETTTS KSTSGLTDIT WSSSGSDLSD EDKTLSQLQR121DELQFIDWEI DSDRAEASDC DEFEDDEGAV EISDCASCAS NQSLTSDEKL SELPKPSSIE181ILEYSSDSEK EDDLENVLLI DSESPHKYHV QFASDARQIM ERLIDPRTKS TETILHTPQK241PTAKFPRTPE NSAKKKLLRG GLAERLNGLQ NRERSAISLW RHQCISYQKT LSGRKSGVLT301VKILELHEEC AMQVAMCEQL LGSPATSSSQ SVAPRPGAGL KVLFTKETAG YLRGRPQDTV361RIFPPWQKLI IPSGSCPVIL NTYFCEKVVA KEDSEKTCEV YCPDIPLPRR SISLAQMFVI421KGLTNNSPEI QVVCSGVATT GTAWTHGHKE AKQRIPTSTP LRDSLLDVVE SQGAASWPGA481GVRVVVQRVY SLPSRDSTRG QQGASSGHTD PAGTRACLLV QDACGMFGEV HLEFTMSKAR541QLEGKSCSLV GMKVLQKVTR GRTAGIFSLI DTLWPPAIPL KTPGRDQPCE EIKTHLPPPA601LCYILTAHPN LGQIDIIDED PIYKLYQPPV TRCLRDILQM NDLGTRCSFY ATVIYQKPQL661KSLLLLEQRE IWLLVTDVTL QTKEERDPRL PKTLLVYVAP LCVLGSEVLE ALAGAAPHSL721FFKDALRDQG RIVCAERTVL LLQKPLLSVV SGASSCELPG PVMLDSLDSA TPVNSICSVQ781GTVVGVDEST AFSWPVCDMC GNGRLEQRPE DRGAFSCGDC SRVVTSPVLK RHLQVFLDCR841SRPQCRVKVK LLQRSISSLL RFAAGEDGSY EVKSVLGKEV GLLNCFVQSV TAHPTSCIGL901EEIELLSAGG ASAEHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
915 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.