LSBio
Human SPIDR / KIAA0146 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPIDR / KIAA0146 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPIDR antibodies →
$220.00each
Catalog Number: LS-G77023-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0146(SPIDR) (NM_001080394) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0146
Origin Species
Human
NCBI GenBank
D63480
SwissProt
Q14159

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MPRGSRARGS KRKRSWNTEC PSFPGERPLQ VRRAGLRTAG AAASLSEAWL RCGEGFQNTS
61GNPSLTAEEK TITEKHLELC PRPKQETTTS KSTSGLTDIT WSSSGSDLSD EDKTLSQLQR
121DELQFIDWEI DSDRAEASDC DEFEDDEGAV EISDCASCAS NQSLTSDEKL SELPKPSSIE
181ILEYSSDSEK EDDLENVLLI DSESPHKYHV QFASDARQIM ERLIDPRTKS TETILHTPQK
241PTAKFPRTPE NSAKKKLLRG GLAERLNGLQ NRERSAISLW RHQCISYQKT LSGRKSGVLT
301VKILELHEEC AMQVAMCEQL LGSPATSSSQ SVAPRPGAGL KVLFTKETAG YLRGRPQDTV
361RIFPPWQKLI IPSGSCPVIL NTYFCEKVVA KEDSEKTCEV YCPDIPLPRR SISLAQMFVI
421KGLTNNSPEI QVVCSGVATT GTAWTHGHKE AKQRIPTSTP LRDSLLDVVE SQGAASWPGA
481GVRVVVQRVY SLPSRDSTRG QQGASSGHTD PAGTRACLLV QDACGMFGEV HLEFTMSKAR
541QLEGKSCSLV GMKVLQKVTR GRTAGIFSLI DTLWPPAIPL KTPGRDQPCE EIKTHLPPPA
601LCYILTAHPN LGQIDIIDED PIYKLYQPPV TRCLRDILQM NDLGTRCSFY ATVIYQKPQL
661KSLLLLEQRE IWLLVTDVTL QTKEERDPRL PKTLLVYVAP LCVLGSEVLE ALAGAAPHSL
721FFKDALRDQG RIVCAERTVL LLQKPLLSVV SGASSCELPG PVMLDSLDSA TPVNSICSVQ
781GTVVGVDEST AFSWPVCDMC GNGRLEQRPE DRGAFSCGDC SRVVTSPVLK RHLQVFLDCR
841SRPQCRVKVK LLQRSISSLL RFAAGEDGSY EVKSVLGKEV GLLNCFVQSV TAHPTSCIGL
901EEIELLSAGG ASAEH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

915 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.