Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPICE1 / CCDC52 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106882-100
Catalog Number: LS-G106882
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC52(SPICE1) (NM_144718), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC52 | SPICE | SPICE1 | Spindle and centriole protein
- Origin Species
- Human
- SwissProt
- Q8N0Z3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSFVRVNRCG PRVGVRKTPK VKKKKTSVKQ EWDNTVTDLT VHRATPEDLV RRHEIHKSKN61RALVHWELQE KALKRKWRKQ KPETLNLEKR RLSIMKEILS DQYQMQDVLE KSDHLIAAAK121ELFPRRRTGF PNVTVAPDSS QGPIVVNQDP ITQSIFNESV IEPQALNDVD GEEEGTVNSQ181SGESENENEL DNSLNSQSNT NTDRFLQQLT EENFELISKL WTDIQQKIAT QSQITPPGTP241SSALSSGEQR AALNATNAVK RLQTRLQPEE STETLDSSYV VGHVLNSRKQ KQLLNKVKRK301PNLHALSKPK KNISSGSTTS ADLPNRTNSN LDVLKHMIHE VEHEMEEYER WTGREVKGLQ361SSQGLTGFTL SLVSSLCRLV RYLKESEIQL RKEVETRQQL EQVLGDHREL IDALTAEILR421LREENAATQA RLQQYMVTTD EQLISLTHAI KNCPVINNRQ EIQASESGAT GRRVMDSPER481PVVNANVSVP LMFREEVAEF PQEELPVKLS QVPDPPDNMN LAKNFPAHIF EPAVLLTPPR541QKSNLKFSPL QDVLRRTVQT RPAPRLPPTV EIIEKEQNWE EKTLPIDTDI QNSSEENRLF601TQRWRVSHMG EDLENKTQAP FVNLSQPLCN SHSNTQQSRS PTFSEELPVL GDGQQLRTNE661SLIQRKDIMT RIADLTLQNS AIKAHMNNII EPRGEQGDGL RELNKQESAS DMTSTFPVAQ721SLTPGSMEER IAELNRQSME ARGKLLQLIE QQKLVGLNLS PPMSPVQLPL RAWTEGAKRT781IEVSIPGAEA PESSKCSTVS PVSGINTRRS SGATGNSCSP LNATSGSGRF TPLNPRAKIE841KQNEEGWFAL STHVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
855 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.