LSBio
Human SPICE1 / CCDC52 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPICE1 / CCDC52 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPICE1 antibodies →
$153.00each
Catalog Number: LS-G93653-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CCDC52(SPICE1) (NM_144718) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCDC52 | SPICE | SPICE1 | Spindle and centriole protein
Origin Species
Human
SwissProt
Q8N0Z3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFVRVNRCG PRVGVRKTPK VKKKKTSVKQ EWDNTVTDLT VHRATPEDLV RRHEIHKSKN
61RALVHWELQE KALKRKWRKQ KPETLNLEKR RLSIMKEILS DQYQMQDVLE KSDHLIAAAK
121ELFPRRRTGF PNVTVAPDSS QGPIVVNQDP ITQSIFNESV IEPQALNDVD GEEEGTVNSQ
181SGESENENEL DNSLNSQSNT NTDRFLQQLT EENFELISKL WTDIQQKIAT QSQITPPGTP
241SSALSSGEQR AALNATNAVK RLQTRLQPEE STETLDSSYV VGHVLNSRKQ KQLLNKVKRK
301PNLHALSKPK KNISSGSTTS ADLPNRTNSN LDVLKHMIHE VEHEMEEYER WTGREVKGLQ
361SSQGLTGFTL SLVSSLCRLV RYLKESEIQL RKEVETRQQL EQVLGDHREL IDALTAEILR
421LREENAATQA RLQQYMVTTD EQLISLTHAI KNCPVINNRQ EIQASESGAT GRRVMDSPER
481PVVNANVSVP LMFREEVAEF PQEELPVKLS QVPDPPDNMN LAKNFPAHIF EPAVLLTPPR
541QKSNLKFSPL QDVLRRTVQT RPAPRLPPTV EIIEKEQNWE EKTLPIDTDI QNSSEENRLF
601TQRWRVSHMG EDLENKTQAP FVNLSQPLCN SHSNTQQSRS PTFSEELPVL GDGQQLRTNE
661SLIQRKDIMT RIADLTLQNS AIKAHMNNII EPRGEQGDGL RELNKQESAS DMTSTFPVAQ
721SLTPGSMEER IAELNRQSME ARGKLLQLIE QQKLVGLNLS PPMSPVQLPL RAWTEGAKRT
781IEVSIPGAEA PESSKCSTVS PVSGINTRRS SGATGNSCSP LNATSGSGRF TPLNPRAKIE
841KQNEEGWFAL STHVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

855 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.