Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPATA18 / MIEAP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106703-100
Catalog Number: LS-G106703
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA18 (NM_145263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Mieap | SPETEX1 | Spermatogenesis associated 18 | Mitochondria-eating protein | SPATA18
- Origin Species
- Human
- SwissProt
- Q8TC71
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAENLKRLVS NETLRTLQEK LDFWLKEYNT NTCDQNLNHC LELIEQVAKV QGQLFGILTA61AAQEGGRNDG VETIKSRLLP WLEASFTAAS LGKSVDSKVP SLQDTFDRER HKDPSPRDRD121MQQLDSNLNS TRSQCNQVQD DLVETEKNLE ESKNRSAISL LAAEEEINQL KKQLKSLQAQ181EDARHRNTDQ RSSENRRSEP WSLEERKREQ WNSLKQNADQ QDTEAMSDYK KQLRNLKEEI241AVLSAEKSAL QGRSSRSRSP SPAPRSRSCS RSRSASPSTA VKVRRPSPNR SKLSNVARKA301ALLSRFSDSY SQARLDAQCL LRRCIDKAET VQRIIYIATV EAFHVAKMAF RHFKIHVRKS361LTPSYVGSND FENAVLDYVI CHLDLYDSQS SVNDVIRAMN VNPKISFPPV VDFCLLSDFI421QEICCIAFAM QALEPPLDIA YGADGEVFND CKYRRSYDSD FTAPLVLYHV WPALMENDCV481IMKGEAVTRR GAFWNSVRSV SRCRSRSLSP ICPRSQIGLN TMSRSRSPSP IRCGLPRFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
538 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.