LSBio
Human SPATA18 / MIEAP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPATA18 / MIEAP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPATA18 antibodies →
$153.00each
Catalog Number: LS-G94477-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPATA18 (NM_145263) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Mieap | SPETEX1 | Spermatogenesis associated 18 | Mitochondria-eating protein | SPATA18
Origin Species
Human
SwissProt
Q8TC71

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MAENLKRLVS NETLRTLQEK LDFWLKEYNT NTCDQNLNHC LELIEQVAKV QGQLFGILTA
61AAQEGGRNDG VETIKSRLLP WLEASFTAAS LGKSVDSKVP SLQDTFDRER HKDPSPRDRD
121MQQLDSNLNS TRSQCNQVQD DLVETEKNLE ESKNRSAISL LAAEEEINQL KKQLKSLQAQ
181EDARHRNTDQ RSSENRRSEP WSLEERKREQ WNSLKQNADQ QDTEAMSDYK KQLRNLKEEI
241AVLSAEKSAL QGRSSRSRSP SPAPRSRSCS RSRSASPSTA VKVRRPSPNR SKLSNVARKA
301ALLSRFSDSY SQARLDAQCL LRRCIDKAET VQRIIYIATV EAFHVAKMAF RHFKIHVRKS
361LTPSYVGSND FENAVLDYVI CHLDLYDSQS SVNDVIRAMN VNPKISFPPV VDFCLLSDFI
421QEICCIAFAM QALEPPLDIA YGADGEVFND CKYRRSYDSD FTAPLVLYHV WPALMENDCV
481IMKGEAVTRR GAFWNSVRSV SRCRSRSLSP ICPRSQIGLN TMSRSRSPSP IRCGLPRF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

538 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.