Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPATA16 / NYD-SP12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102017-100
Catalog Number: LS-G102017
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA16 (NM_031955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SPATA16 | SPGF6 | NYD-SP12 | Spermatogenesis associated 16 | Testis-specific Golgi protein
- Origin Species
- Human
- NCBI GenBank
- NM_031955
- SwissProt
- Q9BXB7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAGSSRSLE NAVNRIYHDQ LVPKINTSKK MSTLAHPPNI LEMSQEIKKN CGGKQVEITL61ERTKMTKGIK EKQSNDLEKA AFKRKAEGEG KPTRKKQAKI TELDNQLITM PLPHIPLKNI121MDVEMKLVYI DEMGVRYEFV ESLMSTGSQP TCQAAEIVDP LSVHNFSFLP QIDKWLQVAL181KDASSCYRQK KYALAAGQFR TALELCSKGA VLGEPFDAPA EDIASVASFI ETKLVTCYLR241MRKPDLALNH AHRSIVLNPA YFRNHLRQAT VFRCLERYSE AARSAMIADY MFWLGGGREE301SISKLIKLYW QAMIEEAITR AESFSVMYTP FATKIRADKI EKVKDAFTKT HPAYAEYMYT361DLQALHMLPQ TVDWSSFPPQ QYLLTLGFKN KDDGKFLEKI SSRKLPIFTE HKTPFGLTRE421DTVRQMETMG KRILPILDFI RSTQLNGSFP ASSGVMEKLQ YASLLSQLQR VKEQSQVINQ481AMAELATIPY LQDISQQEAE LLQSLMADAM DTLEGRRNNN ERVWNMIQKV GQIEDFLYQL541EDSFLKTKKL RTARRQKTKM KRLQTVQQRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
569 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.