LSBio
Human SPATA16 / NYD-SP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SPATA16 / NYD-SP12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all SPATA16 antibodies →
$153.00each
Catalog Number: LS-G92089-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SPATA16 (NM_031955) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SPATA16 | SPGF6 | NYD-SP12 | Spermatogenesis associated 16 | Testis-specific Golgi protein
Origin Species
Human
NCBI GenBank
NM_031955
SwissProt
Q9BXB7

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDAGSSRSLE NAVNRIYHDQ LVPKINTSKK MSTLAHPPNI LEMSQEIKKN CGGKQVEITL
61ERTKMTKGIK EKQSNDLEKA AFKRKAEGEG KPTRKKQAKI TELDNQLITM PLPHIPLKNI
121MDVEMKLVYI DEMGVRYEFV ESLMSTGSQP TCQAAEIVDP LSVHNFSFLP QIDKWLQVAL
181KDASSCYRQK KYALAAGQFR TALELCSKGA VLGEPFDAPA EDIASVASFI ETKLVTCYLR
241MRKPDLALNH AHRSIVLNPA YFRNHLRQAT VFRCLERYSE AARSAMIADY MFWLGGGREE
301SISKLIKLYW QAMIEEAITR AESFSVMYTP FATKIRADKI EKVKDAFTKT HPAYAEYMYT
361DLQALHMLPQ TVDWSSFPPQ QYLLTLGFKN KDDGKFLEKI SSRKLPIFTE HKTPFGLTRE
421DTVRQMETMG KRILPILDFI RSTQLNGSFP ASSGVMEKLQ YASLLSQLQR VKEQSQVINQ
481AMAELATIPY LQDISQQEAE LLQSLMADAM DTLEGRRNNN ERVWNMIQKV GQIEDFLYQL
541EDSFLKTKKL RTARRQKTKM KRLQTVQQR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.