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SP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SP4 antibodies →
$725.00each
Catalog Number: LS-G117544-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transcription factor Sp4(SP4) (NM_003112), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HF1B | Sp4 transcription factor | SPR-1 | SP4 | Transcription factor Sp4
Origin Species
Human
NCBI GenBank
NM_003112
SwissProt
Q02446

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDQKKEEEE EAAAAAAMAT EGGKTSEPEN NNKKPKTSGS QDSQPSPLAL LAATCSKIGT
61PGENQATGQQ QIIIDPSQGL VQLQNQPQQL ELVTTQLAGN AWQLVASTPP ASKENNVSQP
121ASSSSSSSSS NNGSASPTKT KSGNSSTPGQ FQVIQVQNPS GSVQYQVIPQ LQTVEGQQIQ
181INPTSSSSLQ DLQGQIQLIS AGNNQAILTA ANRTASGNIL AQNLANQTVP VQIRPGVSIP
241LQLQTLPGTQ AQVVTTLPIN IGGVTLALPV INNVAAGGGT GQVGQPAATA DSGTSNGNQL
301VSTPTNTTTS ASTMPESPSS STTCTTTAST SLTSSDTLVS SADTGQYAST SASSSERTIE
361ESQTPAATES EAQSSSQLQP NGMQNAQDQS NSLQQVQIVG QPILQQIQIQ QPQQQIIQAI
421PPQSFQLQSG QTIQTIQQQP LQNVQLQAVN PTQVLIRAPT LTPSGQISWQ TVQVQNIQSL
481SNLQVQNAGL SQQLTITPVS SSGGTTLAQI APVAVAGAPI TLNTAQLASV PNLQTVSVAN
541LGAAGVQVQG VPVTITSVAG QQQGQDGVKV QQATIAPVTV AVGGIANATI GAVSPDQLTQ
601VHLQQGQQTS DQEVQPGKRL RRVACSCPNC REGEGRGSNE PGKKKQHICH IEGCGKVYGK
661TSHLRAHLRW HTGERPFICN WMFCGKRFTR SDELQRHRRT HTGEKRFECP ECSKRFMRSD
721HLSKHVKTHQ NKKGGGTALA IVTSGELDSS VTEVLGSPRI VTVAAISQDS NPATPNVSTN
781MEEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

784 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.