Immunofluorescence staining of SP4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP4 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

SP4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$212.00each

Catalog Number: LS-C817334-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: SP4 antibody LS-C817334 is an unconjugated rabbit polyclonal antibody to human SP4. Validated for ICC, IF, IHC and WB.
Application: ICC, IF, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HF1B | Sp4 transcription factor | SPR-1 | SP4 | Transcription factor Sp4
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: IHC-P: 1:200 - 1:1000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q02446
NCBI GenBank: NM_003112 | NP_003103.2
SwissProt: Q02446

Target

Target: Human SP4
Antigen Species: Human
Epitope: Human SP4
Gene Name: SP4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived Human SP4 fragment
Immunogen Type: Recombinant Protein Fragment

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.0, 0.03% Proclin 300
Volume: 50 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS