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SP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117551-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SP2 transcription factor(SP2) (NM_003110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0048 | Sp2 transcription factor | SP2 | Transcription factor Sp2
Origin Species
Human
NCBI GenBank
NM_003110
SwissProt
Q02086

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDPQTSMAA TAAVSPSDYL QPAASTTQDS QPSPLALLAA TCSKIGPPAV EAAVTPPAPP
61QPTPRKLVPI KPAPLPLSPG KNSFGILSSK GNILQIQGSQ LSASYPGGQL VFAIQNPTMI
121NKGTRSNANI QYQAVPQIQA SNSQTIQVQP NLTNQIQIIP GTNQAIITPS PSSHKPVPIK
181PAPIQKSSTT TTPVQSGANV VKLTGGGGNV TLTLPVNNLV NASDTGAPTQ LLTESPPTPL
241SKTNKKARKK SLPASQPPVA VAEQVETVLI ETTADNIIQA GNNLLIVQSP GGGQPAVVQQ
301VQVVPPKAEQ QQVVQIPQQA LRVVQAASAT LPTVPQKPSQ NFQIQAAEPT PTQVYIRTPS
361GEVQTVLVQD SPPATAAATS NTTCSSPASR APHLSGTSKK HSAAILRKER PLPKIAPAGS
421IISLNAAQLA AAAQAMQTIN INGVQVQGVP VTITNTGGQQ QLTVQNVSGN NLTISGLSPT
481QIQLQMEQAL AGETQPGEKR RRMACTCPNC KDGEKRSGEQ GKKKHVCHIP DCGKTFRKTS
541LLRAHVRLHT GERPFVCNWF FCGKRFTRSD ELQRHARTHT GDKRFECAQC QKRFMRSDHL
601TKHYKTHLVT KNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

613 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.