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SMPD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMPD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112111-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMPD4 (NM_017951), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neutral sphingomyelinase 3 | NET13 | NSMASE-3 | Neutral sphingomyelinase III | NSMASE3 | KIAA1418 | SKNY protein | SMPD4
Origin Species
Human
NCBI GenBank
NM_017751

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTFGAVAEW RLPSLRRATL WIPQWFAKKA IFNSPLEAAM AFPHLQQPSF LLASLKADSI
61NKPFAQQCQD LVKVIEDFPA KELHTIFPWL VESIFGSLDG VLVGWNLRCL QGRVNPVEYS
121IVMEFLDPGG PMMKLVYKLQ AEDYKFDFPV SYLPGPVKAS IQECILPDSP LYHNKVQFTP
181TGGLGLNLAL NPFEYYIFFF ALSLITQKPL PVSLHVRTSD CAYFILVDRY LSWFLPTEGS
241VPPPLSSSPG GTSPSPPPRT PAIPFASYGL HHTSLLKRHI SHQTSVNADP ASHEIWRSET
301LLQVFVEMWL HHYSLEMYQK MQSPHAKLEV LHYRLSVSSA LYSPAQPSLQ ALHAYQESFT
361PTEEHVLVVR LLLKHLHAFA NSLKPEQASP SAHSHATSPL EEFKRAAVPR FVQQKLYLFL
421QHCFGHWPLD ASFRAVLEMW LSYLQPWRYA PDKQAPGSDS QPRCVSEKWA PFVQENLLMY
481TKLFVGFLNR ALRTDLVSPK HALMVFRVAK VFAQPNLAEM IQKGEQLFLE PELVIPHRQH
541RLFTAPTFTG SFLSPWPPAV TDASFKVKSH VYSLEGQDCK YTPMFGPEAR TLVLRLAQLI
601TQAKHTAKSI SDQCAESPAG HSFLSWLGFS SMDTNGSYTA NDLDEMGQDS VRKTDEYLEK
661ALEYLRQIFR LSEAQLRQFT LALGTTQDEN GKKQLPDCIV GEDGLILTPL GRYQIINGLR
721RFEIEYQGDP ELQPIRSYEI ASLVRTLFRL SSAINHRFAG QMAALCSRDD FLGSFCRYHL
781TEPGLASRHL LSPVGRRQVA GHTRGPRLSL RFLGSYRTLV SLLLAFFVAS LFCVGPLPCT
841LLLTLGYVLY ASAMTLLTER GKLHQP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

866 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.