SMPD4 Antibody
Catalog Number: LS-C750442
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SMPD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G87711-20
Catalog Number: LS-G87711
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SMPD4 (NM_017951) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Neutral sphingomyelinase 3 | NET13 | NSMASE-3 | Neutral sphingomyelinase III | NSMASE3 | KIAA1418 | SKNY protein | SMPD4
- Origin Species
- Human
- NCBI GenBank
- NM_017751
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 97.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTFGAVAEW RLPSLRRATL WIPQWFAKKA IFNSPLEAAM AFPHLQQPSF LLASLKADSI61NKPFAQQCQD LVKVIEDFPA KELHTIFPWL VESIFGSLDG VLVGWNLRCL QGRVNPVEYS121IVMEFLDPGG PMMKLVYKLQ AEDYKFDFPV SYLPGPVKAS IQECILPDSP LYHNKVQFTP181TGGLGLNLAL NPFEYYIFFF ALSLITQKPL PVSLHVRTSD CAYFILVDRY LSWFLPTEGS241VPPPLSSSPG GTSPSPPPRT PAIPFASYGL HHTSLLKRHI SHQTSVNADP ASHEIWRSET301LLQVFVEMWL HHYSLEMYQK MQSPHAKLEV LHYRLSVSSA LYSPAQPSLQ ALHAYQESFT361PTEEHVLVVR LLLKHLHAFA NSLKPEQASP SAHSHATSPL EEFKRAAVPR FVQQKLYLFL421QHCFGHWPLD ASFRAVLEMW LSYLQPWRYA PDKQAPGSDS QPRCVSEKWA PFVQENLLMY481TKLFVGFLNR ALRTDLVSPK HALMVFRVAK VFAQPNLAEM IQKGEQLFLE PELVIPHRQH541RLFTAPTFTG SFLSPWPPAV TDASFKVKSH VYSLEGQDCK YTPMFGPEAR TLVLRLAQLI601TQAKHTAKSI SDQCAESPAG HSFLSWLGFS SMDTNGSYTA NDLDEMGQDS VRKTDEYLEK661ALEYLRQIFR LSEAQLRQFT LALGTTQDEN GKKQLPDCIV GEDGLILTPL GRYQIINGLR721RFEIEYQGDP ELQPIRSYEI ASLVRTLFRL SSAINHRFAG QMAALCSRDD FLGSFCRYHL781TEPGLASRHL LSPVGRRQVA GHTRGPRLSL RFLGSYRTLV SLLLAFFVAS LFCVGPLPCT841LLLTLGYVLY ASAMTLLTER GKLHQPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
866 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
