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SMEK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMEK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102061-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Smek1(SMEK1) (NM_032560), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLFL1 | KIAA2010 | MSTP033 | SMEK homolog 1 | Smk1 | PP4R3A | PPP4R3A | SMEK1 | Smk-1
Origin Species
Human
NCBI GenBank
NM_032560

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTDTRRRVKV YTLNEDRQWD DRGTGHVSSG YVERLKGMSL LVRAESDGSL LLESKINPNT
61AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QDLVDESEEE
121RFDDMSSPGL ELPSCELSRL EEIAELVASS LPSPLRREKL ALALENEGYI KKLLELFHVC
181EDLENIEGLH HLYEIIKGIF LLNRTALFEV MFSEECIMDV IGCLEYDPAL SQPRKHREFL
241TKTAKFKEVI PISDPELKQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI
301VGMLQEDEKF LTDLFAQLTD EATDEEKRQE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM
361GILPALEVIL GMDDTQVRSA ATDIFSYLVE YNPSMVREFV MQEAQQNDDD ILLINLIIEH
421MICDTDPELG GAVQLMGLLR TLVDPENMLA TANKTEKTEF LGFFYKHCMH VLTAPLLANT
481TEDKPSKDDF QTAQLLALVL ELLTFCVEHH TYHIKNYIIN KDILRRVLVL MASKHAFLAL
541CALRFKRKII GLKDEFYNRY IMKSFLFEPV VKAFLNNGSR YNLMNSAIIE MFEFIRVEDI
601KSLTAHVIEN YWKALEDVDY VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR
661TLEDEEEMWF NTDEDDMEDG EAVVSPSDKT KNDDDIMDPI SKFMERKKLK ESEEKEVLLK
721TNLSGRQSPS FKLSLSSGTK TNLTSQSSTT NLPGSPGSPG SPGSPGSPGS VPKNTSQTAA
781ITTKGGLVGL VDYPDDDEDD DEDEDKEDTL PLSKKAKFDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

820 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.