SMEK1 Antibody
Catalog Number: LS-C668423
4 sizes / from $272.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human SMEK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G84680-20
Catalog Number: LS-G84680
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Smek1(SMEK1) (NM_032560) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLFL1 | KIAA2010 | MSTP033 | SMEK homolog 1 | Smk1 | PP4R3A | PPP4R3A | SMEK1 | Smk-1
- Origin Species
- Human
- NCBI GenBank
- NM_032560
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTDTRRRVKV YTLNEDRQWD DRGTGHVSSG YVERLKGMSL LVRAESDGSL LLESKINPNT61AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QDLVDESEEE121RFDDMSSPGL ELPSCELSRL EEIAELVASS LPSPLRREKL ALALENEGYI KKLLELFHVC181EDLENIEGLH HLYEIIKGIF LLNRTALFEV MFSEECIMDV IGCLEYDPAL SQPRKHREFL241TKTAKFKEVI PISDPELKQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI301VGMLQEDEKF LTDLFAQLTD EATDEEKRQE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM361GILPALEVIL GMDDTQVRSA ATDIFSYLVE YNPSMVREFV MQEAQQNDDD ILLINLIIEH421MICDTDPELG GAVQLMGLLR TLVDPENMLA TANKTEKTEF LGFFYKHCMH VLTAPLLANT481TEDKPSKDDF QTAQLLALVL ELLTFCVEHH TYHIKNYIIN KDILRRVLVL MASKHAFLAL541CALRFKRKII GLKDEFYNRY IMKSFLFEPV VKAFLNNGSR YNLMNSAIIE MFEFIRVEDI601KSLTAHVIEN YWKALEDVDY VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR661TLEDEEEMWF NTDEDDMEDG EAVVSPSDKT KNDDDIMDPI SKFMERKKLK ESEEKEVLLK721TNLSGRQSPS FKLSLSSGTK TNLTSQSSTT NLPGSPGSPG SPGSPGSPGS VPKNTSQTAA781ITTKGGLVGL VDYPDDDEDD DEDEDKEDTL PLSKKAKFDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
820 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
