Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SMARCD1 / BAF60A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99958-100
Catalog Number: LS-G99958
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMARCD1 (NM_003076), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAF60A | BRG1-associated factor 60A | Rsc6p | SMARCD1 | Swp73-like protein | CRACD1 | Swi/snf complex 60 kda subunit
- NCBI GenBank
- NM_003076
- SwissProt
- Q96GM5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.94 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPAPGQGLY RSPMPGAAYP RPGMLPGSRM TPQGPSMGPP GYGGNPSVRP GLAQSGMDQS61RKRPAPQQIQ QVQQQAVQNR NHNAKKKKMA DKILPQRIRE LVPESQAYMD LLAFERKLDQ121TIMRKRLDIQ EALKRPIKQK RKLRIFISNT FNPAKSDAED GEGTVASWEL RVEGRLLEDS181ALSKYDATKQ KRKFSSFFKS LVIELDKDLY GPDNHLVEWH RTATTQETDG FQVKRPGDVN241VRCTVLLMLD YQPPQFKLDP RLARLLGIHT QTRPVIIQAL WQYIKTHKLQ DPHEREFVIC301DKYLQQIFES QRMKFSEIPQ RLHALLMPPE PIIINHVISV DPNDQKKTAC YDIDVEVDDT361LKTQMNSFLL STASQQEIAT LDNKIHETIE TINQLKTQRE FMLSFARDPQ GFINDWLQSQ421CRDLKTMTDV VGNPEEERRA EFYFQPWAQE AVCRYFYSKV QQRRQELEQA LGIRNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
476 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.