LSBio
Human SMARCD1 / BAF60A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SMARCD1 / BAF60A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G81725-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SMARCD1 (NM_003076) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BAF60A | BRG1-associated factor 60A | Rsc6p | SMARCD1 | Swp73-like protein | CRACD1 | Swi/snf complex 60 kda subunit
Origin Species
Human
NCBI GenBank
NM_003076
SwissProt
Q96GM5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPAPGQGLY RSPMPGAAYP RPGMLPGSRM TPQGPSMGPP GYGGNPSVRP GLAQSGMDQS
61RKRPAPQQIQ QVQQQAVQNR NHNAKKKKMA DKILPQRIRE LVPESQAYMD LLAFERKLDQ
121TIMRKRLDIQ EALKRPIKQK RKLRIFISNT FNPAKSDAED GEGTVASWEL RVEGRLLEDS
181ALSKYDATKQ KRKFSSFFKS LVIELDKDLY GPDNHLVEWH RTATTQETDG FQVKRPGDVN
241VRCTVLLMLD YQPPQFKLDP RLARLLGIHT QTRPVIIQAL WQYIKTHKLQ DPHEREFVIC
301DKYLQQIFES QRMKFSEIPQ RLHALLMPPE PIIINHVISV DPNDQKKTAC YDIDVEVDDT
361LKTQMNSFLL STASQQEIAT LDNKIHETIE TINQLKTQRE FMLSFARDPQ GFINDWLQSQ
421CRDLKTMTDV VGNPEEERRA EFYFQPWAQE AVCRYFYSKV QQRRQELEQA LGIRNT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

476 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.