Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLCO1A2 / OATP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102229-100
Catalog Number: LS-G102229
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLCO1A2 (NM_134431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OATP-1 | Oatp1 | OATP1A2 | OATP-A | OATPA | SLCO1A2 | OATP | SLC21A3
- Origin Species
- Human
- NCBI GenBank
- NM_021094
- SwissProt
- P46721
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGETEKRIET HRIRCLFKLK MFLLAITCAF VSKTLSGSYM NSMLTQIERQ FNIPTSLVGF61INGSFEIGNL LLIIFVSYFG TKLHRPIMIG IGCVVMGLGC FLKSLPHFLM NQYEYESTVS121VSGNLSSNSF LCMENGTQIL RPTQDPSECT KEVKSLMWVY VLVGNIVRGM GETPILPLGI181SYIEDFAKFE NSPLYIGLVE TGAIIGPLIG LLLASFCANV YVDTGFVNTD DLIITPTDTR241WVGAWWFGFL ICAGVNVLTA IPFFFLPNTL PKEGLETNAD IIKNENEDKQ KEEVKKEKYG301ITKDFLPFMK SLSCNPIYML FILVSVIQFN AFVNMISFMP KYLEQQYGIS SSDAIFLMGI361YNLPPICIGY IIGGLIMKKF KITVKQAAHI GCWLSLLEYL LYFLSFLMTC ENSSVVGINT421SYEGIPQDLY VENDIFADCN VDCNCPSKIW DPVCGNNGLS YLSACLAGCE TSIGTGINMV481FQNCSCIQTS GNSSAVLGLC DKGPDCSLML QYFLILSAMS SFIYSLAAIP GYMVLLRCMK541SEEKSLGVGL HTFCTRVFAG IPAPIYFGAL MDSTCLHWGT LKCGESGACR IYDSTTFRYI601YLGLPAALRG SSFVPALIIL ILLRKCHLPG ENASSGTELI ETKVKGKENE CKDIYQKSTV661LKDDELKTKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
670 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.